ANTITUMOUR AGENTS FROM MARINE MICROALGAE

这部分建议的长期目标是发展 抗肿瘤、抗病毒、抗微生物药物来源于微藻。 这项建议是根据最近的调查结果， 在甲藻和其他微藻中有希望的化合物。 预期的化合物包括聚醚、大环内酯类、其他 多氧化合物、环肽和缩肽。 那里 强烈地表明，许多独特的代谢物中发现， 海洋无脊椎动物实际上是由微藻进化而来的。 的 申请人的小组在培养微藻方面具有长期的经验， 研究它们的次级代谢产物， 收集未经检验的微藻菌株。 具体地说， L.自由生活的甲藻，硅藻，藻类， 来自温带和热带沃茨的其他微藻， 从淡水中，将被收集，培养和筛选， 生物活性 2.与海洋生物共生或伴生的上述纲的生物 将对无脊椎动物和藻类进行分离、培养和筛选。 3.活性化合物将被分离，并且化合物的结构将被确定。 纯化的化合物将通过物理化学方法进行研究 包括高场核磁共振质谱和X射线 结晶学 抗肿瘤和抗病毒测定以及进一步的 药物的开发将由百时美施贵宝公司进行， 但有限的内部细胞毒性和抗菌试验将 作为快速隔离指南。

The long-term objective of this part of the proposal is to develop antitumor, antiviral, an antimicrobial drugs from microalgal sources. The proposal is based on the recent findings of the occurrence of promising compounds in dinoflagellates and other microalgae. Anticipated compounds include polyethers, macrolides, other polyoxygenated compounds, cyclic peptides and depsipeptides. There are strong indications that many of the unique metabolites found in marine invertebrates are actually derived from microalgae. The applicant's group has lengthy experience in culturing microalgae and studying their secondary metabolites and is in possession of a vast collection of unexamined microalgal strains. Specifically, l. Free-living dinoflagellates, diatoms, algae in Chrysophyta, and other microalgae from both temperate and tropical waters, and some from freshwater, will be collected, cultured and screened for the bioactivity. 2. Organisms of the above classes symbiotic or associated with marine invertebrates and algae will be isolated, cultured and screened. 3. Active compounds will be isolated, and the structures of the purified compounds will be studied by physico-chemical methods including high field NMR, mass spectroscopy, and X-ray crystallography. Antitumor and antiviral assays and further development of drugs will be conducted by Bristol-Myers Squibb Co., but limited in-house cytotoxicity and antimicrobial tests will be performed as quick isolation guides.