ANTITUMOR, ANTIVIRAL AGENTS IN MICROALGAE

微藻中的抗肿瘤、抗病毒剂

• 批准号: 3194364
• 负责人: YUZURU SHIMIZU
• 金额: $ 18.45万
• 依托单位: UNIVERSITY OF RHODE ISLAND
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-06-01 至 1994-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3194364
• 关键词: Mastigophora; X ray crystallography; algae; antiinfective agents; antineoplastics; antiviral agents; cytotoxicity; mass spectrometry; medicinal plants; microorganism culture; nuclear magnetic resonance spectroscopy

This proposal is to investigate microalgae, especially dinoflagellates, for novel antitumor, antiviral and antimicrobial agents. The research is also intended to determine if these microalgae are the primary sources of highly active interesting molecules recently discovered in marine invertebrates, and thus can be reliable sources of these potentially important therapeutic agents. Anticipated compounds include polyethers, macrolides, and other polyoxygenated compounds. The proposal is based on the recent findings of the occurrence of these compounds in dinoflagellates. Specifically, both free-living and symbiotic or associated organisms will be isolated and cultured in large scale. Emphasis will be on freshly isolated strains from natural habitats or hosts, although those held in culture collections and already in our possession will be also investigated. The cells and broths will be extracted, and the extracts will be fractionated guided by bioassays. The structures of purified compounds will be studied by physico-chemical methods including high field NMR, mass spectroscopy, and X-ray crystallography. Antitumor and antiviral assays will be conducted by Bristol-Myers Co., and NCI, and in- house cytotoxicity tests will be performed with limited cell lines as routine isolation guides. Antimicrobial tests will be done in house.

这项建议是调查微藻，特别是腰鞭毛虫， 用于新型抗肿瘤、抗病毒和抗微生物剂。 这项研究是 还打算确定这些微藻是否是 最近在海洋中发现的高活性有趣分子 无脊椎动物，因此可以成为这些潜在的可靠来源 重要的治疗药物。 预期的化合物包括聚醚， 大环内酯和其它多氧化合物。 所涉提议主要是 最近发现这些化合物在 腰鞭毛虫 具体地说，自由生活和共生或相关的生物体都将 大规模分离培养。 重点将放在新鲜 从自然栖息地或宿主中分离的菌株，尽管那些在 文化收藏和已经在我们拥有的也将是 研究了 将提取细胞和肉汤，提取物 将在生物测定的指导下进行分级。 纯化的结构 化合物将通过物理化学方法进行研究，包括高 场核磁共振质谱和X射线晶体学 抗肿瘤和 抗病毒试验将由Bristol-Myers公司进行，和NCI，在- 将使用有限细胞系进行室内细胞毒性试验， 常规隔离指南。 抗菌测试将在室内进行。