CORE--CELL CULTURE FACILITY

组织库核心机构将参与采集 和分配前列腺组织的成员的孢子赠款。 组织库核心设施有以下目标：（1）最大限度地 所采集的组织的量，同时仍然能够彻底 标本的病理学评估。(2)获得与 可能（即肿瘤由>75%的肿瘤细胞核组成，或良性） 前列腺组织无明显污染 浸润性癌(3)获得具有广谱 分级、分期和组织学亚型。 (4)获得不同类型的 良性前列腺组织（移行区和外周区）。（五） 收获含有推定前列腺前驱病变的组织 癌症（前列腺上皮内瘤）。(6)协调 向SPORE赠款的不同成员分发组织， 最后是机构外的研究人员。 双方都将 由肿瘤、良性外周带、良性移行带组成， 精囊和肿瘤附近组织。 新颖技术 已经发展到可以获取最多的组织 而不损害标本的病理学评估。 准确的病理分期和切缘评估 对于辅助治疗试验和 各种研究参数与肿瘤的相关性 生物行为 前列腺和相关组织的冷冻样本 共收集了658名个体， 自1995年1月12日起在约翰霍普金斯医院接受手术。 1993年，我们成立了 研究人员可以通过这个系统获得新鲜无菌的前列腺 以及冷冻的前列腺组织。 因为只有 可以收获相对少量的癌组织， 研究使用从每个前列腺，组织提供给研究人员 在加权轮换的基础上。 使用该系统，381个新鲜组织 已向187名个人中的14名提供了样本， 自从我们开始跟踪这一点以来， 前列腺组织库样本编号338 成为优先关注 病毒转导研究，因为他们需要大量的 肿瘤样本，其余的研究人员大致相同。 冷冻组织已被用于突变研究，等位基因丢失 原位杂交研究，原位PCR研究， 免疫化学研究和端粒酶研究，由几个（超过 10）独立的研究小组。

The Tissue Bank Core Facility will be involved in the harvesting and distribution of prostatic tissue to members of the SPORE grant. The Tissue Bank Core Facility has the following aims: (1) Maximize the amount of tissue harvested while still enabling thorough pathologic assessment of the specimen. (2) Obtain as pure tissue as possible (i.e. tumor composed of >75% tumor nuclei, or benign prostate tissue without contamination by grossly inapparent infiltrating cancer. (3) Obtain tumors with a wide spectrum of grade, stage and histologic subtype. (4) Obtain different types of benign prostate tissue (transitional and peripheral zone). (5) Harvest tissue containing putative precursor lesions of prostate cancer (prostatic intraepithelial neoplasia). (6) Coordinate the distribution of tissue to varies members of the SPORE grant and eventually to researchers outside of the institution. Each will consist of tumor, benign peripheral zone, benign transition zone, seminal vesicles, and tissue adjacent to tumor. Novel techniques have been developed to harvest the greatest amount of tissue without compromising the pathologic assessment of the specimen. Accurate pathologic staging and evaluation of margins of resection will be necessary for adjuvant therapeutic trials and for correlation of various investigational parameters with tumor biologic behavior. Frozen samples of prostate and related tissue have been collected from a total of 658 individuals undergoing surgery at Johns Hopkins Hospital as of 1/12/95. In 1993 we set up a system whereby researchers can obtain fresh, sterile prostate tissue, as well as frozen prostate tissue. Because only a relatively small amount of cancer tissue can be harvested for research use from each prostate, tissue is provided to researchers on a weighted rotating basis. Using this system, 381 fresh tissue samples have been provided to 14 separate individuals from 187 separate patients since we began keeping track of this with prostate tissue bank sample number 338. Priority has been given to the viral transduction studies because of their need for large tumor samples, with the remaining researchers roughly at par. Frozen tissue has been used for mutation studies, allelic loss studies, in situ hybridization studies, in situ PCR studies, immunochemistry studies, and telomerase studies by several (over ten) separate research groups.