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CORE--MORPHOLOGY LABORATORY

CORE--MORPHOLOGY LABORATORY
核心--形态实验室
批准号:
6238946
负责人:
KENT John JOHNSON
金额:
$4.83万
依托单位国家:
美国
项目类别:
财政年份:
1997
资助国家:
美国
项目状态:
已结题
起止时间:
1997-08-01 至 1998-07-31

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中文摘要
翻译
形态学核心为肾脏的所有部分提供支持 通过提供细胞和组织的常规处理, 以及形态学和形态测量学解释。 纳入本 核心是冷冻组织的切片和处理设施 和用于免疫组织化学的细胞,包括免疫过氧化物酶和 免疫荧光 此外,该核心还规定, 光学显微镜用石蜡包埋组织的处理和分析 包括特殊菌株。 最后,但并非最不重要的是,核心规定, 用超微结构对组织和细胞进行常规处理和分析 (EM)分析. 如果适用,则进行定量形态测定分析。 也完成了。 从历史上看,肾脏中心的形态核心受到了严重的影响, 每年从所有部门提交数百份样品 的中心。 我们预计,这种大量使用将继续下去, 事实增加。 例如,我们预计, 更新期将处理155个冷冻切片; 825个样本 处理的光学显微镜,和535个标本的EM分析。 因此,如可以理解的,形态核心提供了基本的 为肾脏中心的所有研究者提供服务。
英文摘要
The morphology core provides support for all sections of the renal center by providing for the routine processing of cells and tissues as well as morphologic and morphometric interpretation. Included in this core are facilities for the sectioning and processing of frozen tissue and cells for immunohistochemistry including immunoperoxidase and immunofluorescence. In addition, this core also provides for the processing and analysis of paraffin embedded tissue for light microscopy including special strains. Last, but not least, the core provides for routine processing and analysis of tissues and cells by ultrastructural (EM) analysis. If applicable, quantitative morphometric analysis is also done. Historically, the morphology core of the renal center has received heavy usage with several hundred samples submitted each year from all sections of the center. We anticipate that this heavy usage will continue and in fact increase. For example, we project that for the first year of the renewal period that 155 frozen sections will be processed; 825 samples processed for light microscopy, and 535 specimens for EM analysis. Thus, as can be appreciated, the morphology core provides an essential service for all the investigators in the renal center.
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CORE--Morphology Core
CORE--MORPHOLOGY
CORE--MORPHOLOGY
CORE--MORPHOLOGY
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