CORE--MHC MARKERS AND CELL CULTURE FACILITY

这个核心是一个组织培养、蛋白质纯化和MHC分型的核心。 在这个设施中，我们执行所有的样品处理，包括 外周血淋巴细胞的分离、冷冻保存、 冷冻细胞的解冻、洗涤及B细胞系的制备。这个 CORE还执行以下测试：通过血清学对人类白细胞抗原A、B、C进行分型 所有新样本以及少数选定样本的人类白细胞抗原-DR、DQ血清学。 此外，核心使用的是人类白细胞抗原II类基因的DNA分型 序列特异性寡核苷酸探针杂交(SSOPH)。其他 补体蛋白分析等分型也在新的 样本。当出现以下情况时，核心还会对其他标记执行其他测试 确认重组事件所必需的。核心将负责 超抗原和部分单抗的提纯 在一些项目中使用了抗体。

This Core is a tissue culture, protein purification and MHC typing core. In this facility we perform all sample processing that includes the following: isolation of peripheral blood lymphocytes, cryopreservation, thawing and washing of frozen cells and preparation of B cell lines. The core also performs the following tests: HLA-A, B, C typing by serology on all new samples as well as HLA-DR, DQ serology on a few selected samples. In addition, the core does DNA typing for HLA Class II alleles using sequence specific oligonucleotide probe hybridization (SSOPH). Other typings such as complement protein analysis are also performed on new samples. The Core also performs additional tests for other markers when necessary to confirm recombinant events. The Core will be responsible for the purification of the superantigens and some of the monoclonal antibodies used in some of the projects.