PURCHASE A MICROCAL DIFFERENTIAL SCANNING CALORIMETER

要求提供资金，以购置一台高灵敏度微量热计， 差示扫描量热法和等温 NCRR共享仪器资助下的滴定量热法 程序.这种微量热计将被放置在一个生物分子 认可核心融资机制，将提供给主要 下列用户及其实验室成员，以及 许可证，在大学的其他教师。大学 密歇根州目前缺乏高灵敏度的仪器 差示扫描量热法，没有仪器能够 等温滴定量热法因此，所要求的设备将 为NIH资助的广泛的 生物化学系的教员， 生物物理学和化学。该设备将用于获得 大分子及其相互作用的热力学信息 配体将补充正在进行的结构和机制 蛋白质、寡糖和寡核苷酸的表征。 所要求的设备的一个主要用途是评估 构象转变和局部的能量影响 蛋白质的相互作用蛋白质及其相互作用 配体，包括碳水化合物和寡核苷酸，也将被 使用等温滴定量热法研究。最后，差分 用扫描量热法研究了热变性 以及蛋白质和寡核苷酸的复性。能够使用 量热技术将大大加强的能力， 主要用户解决目前正在研究的问题， 实验室，并赞助一个短期课程， 生物分子识别倡议的微量热法将 为这些教师提供微量热法的培训机会 以及他们的学生和研究员

Funds are requested for a high sensitivity microcalorimeter with capabilities for differential scanning calorimetry and isothermal titration calorimetry under the NCRR Shared Instrumentation Grant Program. This microcalorimeter will be placed in a Biomolecular Recognition Core Facility where it will be available to the major users listed below and members of their laboratories, and as time permits, to other faculty in the University. The University of Michigan presently lacks instrumentation for high sensitivity differential scanning calorimetry and has no instrument capable of isothermal titration calorimetry. Thus the requested equipment will provide entirely new capabilities for a broad spectrum of NIH-funded faculty members in the Departments of Biological Chemistry, Biophysics, and Chemistry. The equipment will be used to obtain thermodynamic information on macromolecules and their interactions with ligands that will complement ongoing structural and mechanistic characterizations of proteins, oligosaccharides and oligonucleotide. A major use of the requested equipment will be to assess the energetic implications of conformational transitions and local interactions in proteins. Interactions between proteins and their ligands, including carbohydrates and oligonudeotides, will also be studied using isothermal titration calorimetry. Finally, differential scanning calorimetry will be used to study the thermal denaturation and renaturation of proteins and oligonucleotide. The ability to use calorimetric techniques will greatly strengthen the ability of the major users to address problems currently under study in their laboratories, and the sponsorship of a short course in microcalorimetry by the Initiative in Biomolecular Recognition will provide training opportunities in microcalorimetry for these faculty and their students and fellows.