A microcalorimetry system to define the biochemical parameters of molecular interactions

用于定义分子相互作用的生化参数的微量热系统

• 批准号: RTI-2016-00436
• 负责人: Affar, ElBachir
• 金额: $ 6.72万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=591438
• 关键词: microcalorimetry; system; define; biochemical; parameters

The physico-chemical driving forces of macromolecular interactions dictate all cellular processes in all living organisms, and as such constitute fundamental principles of primary importance for a wide range of research topics including epigenetics and chromatin biology, DNA repair and genomic stability, immunology, microbiology, cell signaling and reproduction. Moreover, understanding macromolecular interactions is crucial for many practical applications including molecular engineering in bioindustry and drug discovery in pharmaceutical research. Calorimetry has become the method of choice for rigorously characterizing molecular interactions and defining their thermodynamic parameters. The principle of calorimetric analyses is based on the notion that the amount of heat absorbed or released when the biomolecules of interest interact can be captured and translated into interaction data including affinity and stoichiometry. This data can be used to draw conclusions about the importance of processes. The TA Instruments NanoITC is a very powerful tool that can be used to accurately and efficiently perform these important measurements. The NanoITC instrument is configured to ensure high-sensitivity analyses on nanomolar quantities of biomolecules thus increasing laboratory productivity and efficiency. This functionality is ensured through a combination of a high sensitivity calorimeter, highly precise and very stable temperature control and efficient titrant delivery. We are a group of 10 researchers that need this technology to catalyze the prompt execution of our research programs and ensure that, during the next 6 years, several hundreds of trainees will acquire the expertise, thus maintaining our world-class leadership in the life sciences and molecular engineering.

大分子相互作用的物理化学驱动力决定了所有生物的所有细胞过程，因此构成了一系列研究课题的首要原则，包括表观遗传学和染色质生物学、DNA修复和基因组稳定性、免疫学、微生物学、细胞信号和生殖。此外，了解大分子相互作用对于许多实际应用是至关重要的，包括生物工业中的分子工程和制药研究中的药物发现。量热法已成为严格表征分子相互作用和确定其热力学参数的首选方法。量热分析的原理是基于这样一个概念，即当感兴趣的生物分子相互作用时，吸收或释放的热量可以被捕获并转化为相互作用数据，包括亲和力和化学计量。这些数据可以用来得出关于过程重要性的结论。TA Instruments NanoITC是一个非常强大的工具，可以用来准确和高效地执行这些重要的测量。NanoITC仪器的配置确保了对纳摩尔量生物分子的高灵敏度分析，从而提高了实验室的生产率和效率。高灵敏度的量热仪、高精度且非常稳定的温度控制和高效的滴定剂输送相结合，确保了这一功能。我们是一个由10名研究人员组成的团队，他们需要这项技术来催化我们的研究计划的迅速执行，并确保在未来6年内，数百名学员将获得专业知识，从而保持我们在生命科学和分子工程领域的世界级领先地位。