A microcalorimetry system to define the biochemical parameters of molecular interactions

大分子相互作用的物理化学驱动力决定了所有活生物体中的所有细胞过程，因此构成了广泛研究主题的首要基本原则，包括表观遗传学和染色质生物学，DNA修复和基因组稳定性，免疫学，微生物学，细胞信号传导和生殖。此外，了解大分子相互作用对于许多实际应用至关重要，包括生物工业中的分子工程和药物研究中的药物发现。量热法已成为严格表征分子相互作用和确定其热力学参数的首选方法。量热分析的原理是基于这样的概念，即当感兴趣的生物分子相互作用时吸收或释放的热量可以被捕获并转化为相互作用数据，包括亲和力和化学计量。这些数据可用于得出有关流程重要性的结论。TA Instruments NanoITC是一款非常强大的工具，可用于准确高效地执行这些重要的测量。NanoITC仪器的配置可确保对纳摩尔量的生物分子进行高灵敏度分析，从而提高实验室的生产力和效率。通过高灵敏度量热计、高精度和非常稳定的温度控制以及高效的滴定剂输送，确保了这一功能。我们是一个由10名研究人员组成的团队，他们需要这项技术来促进我们研究项目的迅速执行，并确保在未来6年内，数百名学员将获得专业知识，从而保持我们在生命科学和分子工程领域的世界级领导地位。

The physico-chemical driving forces of macromolecular interactions dictate all cellular processes in all living organisms, and as such constitute fundamental principles of primary importance for a wide range of research topics including epigenetics and chromatin biology, DNA repair and genomic stability, immunology, microbiology, cell signaling and reproduction. Moreover, understanding macromolecular interactions is crucial for many practical applications including molecular engineering in bioindustry and drug discovery in pharmaceutical research. Calorimetry has become the method of choice for rigorously characterizing molecular interactions and defining their thermodynamic parameters. The principle of calorimetric analyses is based on the notion that the amount of heat absorbed or released when the biomolecules of interest interact can be captured and translated into interaction data including affinity and stoichiometry. This data can be used to draw conclusions about the importance of processes. The TA Instruments NanoITC is a very powerful tool that can be used to accurately and efficiently perform these important measurements. The NanoITC instrument is configured to ensure high-sensitivity analyses on nanomolar quantities of biomolecules thus increasing laboratory productivity and efficiency. This functionality is ensured through a combination of a high sensitivity calorimeter, highly precise and very stable temperature control and efficient titrant delivery. We are a group of 10 researchers that need this technology to catalyze the prompt execution of our research programs and ensure that, during the next 6 years, several hundreds of trainees will acquire the expertise, thus maintaining our world-class leadership in the life sciences and molecular engineering.