CORE--ANALYTIC CORE

核心分析实验室负责量化 广泛的调解人，这些调解人可能在 肺功能障碍的发展。核心实验室在以下方面提供专业知识 所有形式的层析，定量质谱学， 放射免疫分析和酶联免疫吸附试验。每个项目生成的样本将是 运输到核心实验室进行存储和后续分析，从而 以最大限度地利用人员和降低用品成本。 建议的分析方法包括脂类介体(TxB2，6-酮-2)。 前列腺素F1α、前列腺素E_2、花生四烯酸、低密度脂蛋白和尿TxB2代谢物， 和前列环素)，细胞因子(肿瘤坏死因子、IL-1、IL-6和IL-8)， 内皮素、谷胱甘肽和报告基因分析(氯霉素 乙酰转移酶)。该核心实验室每年将处理5000多个样品 年，促使与SCOR调查人员在几乎 并强调了持续质量保证的必要性。

The Core Analytical Laboratory has responsibility for quantification of a broad range of mediators that may prove to be pivotally important in the development of lung dysfunction. The Core Lab provides expertise in all forms of chromatography, quantitative mass spectrometry, radioimmunoassay and ELISA. Samples generated by each project will be transported to the Core Lab for storage and subsequent analysis, allowing for optimal use of personnel and a reduction in cost of supplies. Proposed assays include those for lipid mediators (TxB2, 6-keto- PGF1alpha, PGE2, arachidonic acid, LTB, and urinary metabolites of TxB2, and prostacyclin), cytokines (TNF, IL-1, IL-6 and IL-8), endothelin,glutathione, and reporter gene assays (chloramphenicol acetyltransferase). This Core Lab will process over 5000 samples per year, prompting interaction with the SCOR investigators on an almost daily basis and underscoring the need for ongoing quality assurance.