92 KDA TYPE IV COLLAGENASE INCREASES DURING EXPERIMENTAL INTRAUTERINE INFECTION

产妇生殖道感染与 胎膜早破与早产的关系 劳力。已有研究表明，感染会触发一种细胞因子 参与胎膜早破发病的级联反应。最近，我们 基质金属蛋白酶(MMPs)在卵巢癌组织中的过度表达 肿瘤坏死因子-`诱导人羊膜源性细胞。 MMPs在绒毛膜中的表达增加被认为是一种 舞会的机制。三只怀孕的恒河猴被慢性 于妊娠第120天插管。感染是通过以下方式确定的 经绒毛蜕膜导管接种B组链球菌。 连续采集前、后羊水样本 细菌接种。样品用凝胶底物分析。 酶谱和蛋白质印迹。92 kDa的无活性形式和83 kDa的活性形式 感染后24小时羊水中有基质金属蛋白酶9的表达。这个 92 kDa和83 kDa形式的量/活性与 接种剂量，当羊水中存在细菌时，接种量更高 流体。高分子量二聚体的存在是其特征 感染启动的基质金属蛋白酶-9诱导。72 kDa-IV型血清白蛋白水平 胶原酶与金属蛋白酶组织抑制物 (TIMP-1)不受感染的影响。这些发现支持 羊水内感染和羊水过少之间存在分子联系 毕业舞会。存在宫内感染和羊水增加 细胞因子与较高数量的基质金属蛋白酶-9相关。增强的 细胞外基质降解会使绒毛羊膜变弱 最后是毕业舞会的出现。

Maternal genital tract infections have been associated with the development of premature rupture of the membranes (PROM) and preterm labor. It has been suggested that infection triggers a cytokine cascade that participates in the pathogenesis of PROM. Recently, we demonstrated the overexpression of matrix metalloproteinases (MMPs) in human amnion-derived cells induced by tumor necrosis factor (TNF-`). Increased expression of MMPs in chorioamnion has been proposed as a mechanism of PROM. Three pregnant rhesus monkeys were chronically catheterized at day 120 of gestation. Infection was established by inoculation of group B streptococci through a choriodecidual catheter. Amniotic fluid (AF) samples were collected serially before and after bacterial inoculation. Samples were analyzed by gel-substrate zymography and Western blot. A 92 kDa inactive form and 83 kDa active MMP-9 appeared in amniotic fluid 24 hr following infection. The amount/activity of 92 and 83 kDa forms showed a dependance on the inoculum dose and was higher when bacteria were present in amniotic fluid. Presence of high molecular weight dimers was characteristic of infection-initiated MMP-9 induction. The levels of 72 kDa type-IV collagenase (MMP-2) and tissue inhibitor of metalloproteinases (TIMP-1) were not modified by infection. These findings support the existence of a molecular link between intraamniotic infection and PROM. Presence of intrauterine infection and increased amniotic fluid cytokines correlates with higher amounts of MMP-9. Augmented extracellular matrix degradation will condition chorioamnion weakening and eventually the appearance of PROM.