MOLECULAR BIOLOGY OF BLOOD COAGULATION FACTORS

这项研究的长期目标是建立分子机制 是调节动态平衡系统的基础。总的血液 随着年龄的增长，凝血潜能显著增加。这可能已经 血栓形成频率增加的重要临床意义 以及随着年龄的增长而出现的心血管疾病。作为第一个模式基因 为了让我们深入了解潜在的机制，我们分析了 大脑皮层结构与年龄相关调节的关系 用转基因小鼠表达因子IX基因。我们已经成功地确定了 该基因的一个小的5‘上游区域(约400bp)是 不仅对其与年龄相关的监管至关重要，而且对其 严密的肝脏特异性表达。该地区是1号线的一部分 重复序列，从而确定了新的生物学功能 重复的序列。

The long-term goal of this study is to establish the molecular mechanism underlying the regulation of the homeostasis system. The overall blood coagulation potential dramatically increases with aging. This may have a great clinical significance in the increased frequency of thrombotic and cardiovascular disorders with advancing age. As a first model gene for us to gain insights into the underlying mechanism, we have analyzed the relationship of the structure and age-associated regulation of the factor IX gene with transgenic mice. We have successfully identified a small 5' upstream region (approximately 400 bp) of the gene to be critical not only for its age-associated regulation, but also for its tight liver specific expression. This region is a part of the Line 1 repetitive sequence, thus identifying the novel biological functions for the repetitive sequence.