STRUCTURE ELUCIDATION OF PROTEINS BY MASS SPECTROMETRY

在结构生物学研究所，史密斯教授将把时间分配在三个不同的活动中。 大约三分之一的时间将用于休假的主要目标，与结构生物学家使用X射线晶体学，核磁共振，低温电子显微镜和分子生物学进行互动。 他还将花费大约三分之一的时间共同指导一名研究生（Florestan Desmaris）和一名博士后研究助理（Jean Armengaud博士）的研究。 两者都使用HX MS来检测蛋白质的构象变化，并在IBS蛋白质质谱仪主任Eric Forest博士的监督下进行。 预计该活动将成为就HX MS方法学的实验细节进行富有成效的交流的基础。 史密斯教授的努力的其余三分之一将被要求指导他正在进行的研究计划在HX MS在内布拉斯加大学。 该研究小组由大约10名研究生，4名博士后研究助理和3名长期工作人员组成，主要由两项NIH赠款（ROI GM 40384和RO1 EY07609）。

While at the Institut de Biologie Structurale, Prof Smith will divide his time among three different activities. Approximately one third of his time will be devoted to the principal goal of the sabbatical leave, interacting with structural biologists using X-ray crystallography, nuclear magnetic resonance, cryo- electron microscopy and molecular biology. He will also spend approximately one third of his time co-directing the research of one graduate student (Florestan Desmaris) and one postdoctoral research associate (Dr. Jean Armengaud). Both use HX MS to detect conformational changes in proteins and are under the supervision of Dr. Eric Forest, Director to of the Laboratoire de Spectrometrie de Masse des Proteines at IBS. This activity is expected to be the basis for a fruitful exchange on experimental details of HX MS methodology. The remaining third of Prof. Smith's effort will be required to direct his on-going research program in HX MS at the University of Nebraska. This research group, which consists of approximately 10 graduate students, four postdoctoral research associates and three permanent staff, is funded principally by two NIH grants (ROI GM 40384 and RO1 EY07609).