PILOT--SYSTEM FOR SIMULTANEOUS OPTICAL AND PATCH CLAMP RECORDING FROM HEPATOCYTES

我们建议开发一种新的基于计算机的系统， 单细胞同步视频成像和膜片钳记录 和对联 尽管存在用于以下各项功能的系统： 荧光成像、膜片钳电流记录和电容 测量，这些测量的同步和集成 在一个实验系统中尚未实现。 我们建议 分三个阶段建立这样一个系统。 在这份提案中，我们要求 前两个阶段的资金，其中涉及设计和 将控制和同步数据的软件的实施 实验收集和分析： 1. 软件设计与膜片钳接口。 第一年，我们 我打算规划软件的体系结构，并根据现有的 基于macintosh的膜片钳采集程序，用 QuickTime同步系统。 2. 实现定量图像分析。 工作在第二 一年将是适应模块，执行荧光比 和形态图像处理在QuickTime框架中工作。 这些软件开发构成了第三阶段的基础， 实际实验装置的构建。

We propose to develop a novel computer-based system for performing simultaneous video imaging and patch-clamp recording from single cells and couplets. Although systems exist for the individual functions of fluorescence imaging, patch-clamp current recording and capacitance measurement, the synchronization and integration of these measurements in one experimental system has not been accomplished. We propose to develop such a system in three stages. In this proposal we request funding for the first two stages, which involve the design and implementation of the software that will control and synchronize the data collection and analysis for experiments: 1. Software design and patch-clamp interface. In the first year we intend to plan the architecture of the software, and to adapt as existing Macintosh-based patch-clamp acquisition program to work with the QuickTime synchronization system. 2. Implementation of quantitative image analysis. Work in the second year will be the adaptation of modules that perform fluorescence ratio and morphometric image processing to work in the QuickTime framework. These software developments form the basis for the third stage, the construction of the actual experimental setup.