ROLE OF MILK-BORNE SUBSTANCES IN DEVELOPING LIVER

这项研究的总体目标是阐明结构和功能 微血管及其周围发育的差异 乳鼠肝脏中的实质和细胞可能属于 母乳中含有荷尔蒙或类似激素的物质。这 是使用高分辨率的体内显微方法完成的，如 以及光镜和电子显微镜下的组织学形态计量学， 免疫组织化学和原位杂交准备。更多 具体地说，将研究以下参数作为以下参数的函数 人工奶日粮中单独存在或不存在生长因子 或其组合，例如表皮生长因子(EGF)、催乳素和 胰岛素样生长因子(IGF I和IGF II) 对哺乳期大鼠肝脏的生物完整性：(A)发育 肝脏微血管系统，包括血流模式和速度， 血管的直径、长度和间隔的尺寸变化 对测试物质的反应性；(B)正弦波壁的形成 结构，包括细胞组成以及异质性和 功能；(C)肝实质细胞组织发育成 成体细胞板由DNA合成指数、有丝分裂指数、 出现双核细胞和结构异质性；(D) 肝脏糖代谢功能异质性的研究进展 实质细胞在血管内皮细胞生长因子中的表达 门静脉周围酶、葡萄糖-6-磷酸酶G-6-Pase和小叶中心 经原位杂交证实为葡糖激酶(GK)。这个 研究结果应该有助于阐明这些物质(S)在 在哺乳期影响出生后肝脏发育。这个 这些结果最终也可能有助于建立 在专有婴儿配方奶粉中加入其中一些物质。

The overall aim of this study is to elucidate structural and functional differences in the development of the microvasculature and its surrounding parenchyma and cells in livers of suckling rats that may be attributed to the presence of hormones or hormone-like substances in breast milk. This is being accomplished using high resolution in vivo microscopic methods as well as light and electron microscopic morphometry of histologic, immunohistochemical, and in situ hybridization preparations. More specifically, the following parameters will be studied as a function of the presence or absence in an artificial milk diet of growth factors alone or in combination, e.g., epidermal growth factor (EGF), prolactin, and insulin-like growth factors (IGF I and II), which are transported biologically intact to the liver of suckling rats: (a) development of the hepatic microvascular system including patterns and rates of blood flow, dimensional changes in diameters, lengths and separation of vessels and reactivity to the test substances; (b) development of sinusoid wall structure including cellular composition as well as heterogeneity and function; (c) development of hepatic parenchymal cell organization into adult cell plates as reflected by DNA synthetic index, mitotic index, appearance of binucleated cells and structural heterogeneity; (d) development of functional heterogeneity for glucose metabolism in hepatic parenchymal cells as reflected in the expression of mRNA for the periportal enzyme, glucose-6-phosphatase G-6-Pase, and the centrilobular enzyme, glucokinase (GK), demonstrated by in situ hybridization. The results should help to elucidate the role(s) of these substances in affecting postnatal liver development during the suckling period. The results also ultimately may be useful in establishing the rationals for inclusion of some of these substances in proprietary infant milk formulas.