ION GRADIENTS AND METABOLIC ENERGY IN ANIMAL TISSUE

我们早些时候已经证明了无机物的梯度 细胞内相和胞外相之间的离子，以及与 电池的水分布和电势可以是 仅仅通过改变为细胞的代谢底物而改变。一个 例如肝细胞静息电势的变化。 当肝脏代谢酒精时引起的(Veech，R.L.et 艾尔，酒精中毒，克莱恩。外挂。种马。18：1040-1056,1994)。这些 洞察力可以扩展到对重伤的急救 病人。当细胞受到任何方式的伤害时，它会立即 经历了一系列刻板的反应，在那里它获得了钠 和钙，失去钾，肿胀。这种反应会发生 因为活细胞构成了一个Gibbs-Donnan系统 主要无机离子的梯度接近平衡。 随着三磷酸腺苷的水解能量，电势之间 这些阶段和彼此(Masuda，T.等人，J.Biol.Chem.265： 20321-20334,1990年)。我们现在已经证明了简单的新陈代谢 手段可能改变ATP水解的能量(Kashiwaya，Y.等人， 上午好。J·卡迪尔。80：50A-64A，1997)，并以此恢复 严重创伤患者体内无机离子的正常梯度 伯恩斯。通过改变替代疗法将代谢也包括在内 作为简单的体积考虑，应该能够减少 急性出血和烧伤的发病率和死亡率。这些 概念将是将在学院举行的会议的主题 医学部将于1998年9月16日至18日举行。

We had earlier shown that the gradients of inorganic ions between the intra and extracellular phases of the cell, and with that the water distribution and electrical potential of the cell can be changed simply by changing to cell's metabolic substrate. An example is the change in resting electrical potential of hepatocytes brought about when the liver metabolizes ethanol (Veech, R.L. et al, Alcoholism, Clin. Exptl. Stud. 18: 1040-1056, 1994). These insights can be extended to the acute treatment of severely injured patients. When a cell is injured by any means, it immediately undergoes a series of stereotyped reactions where it gains sodium and calcium, looses potassium and swells. This reaction occurs because the living cell constitutes a Gibbs-Donnan system in which the gradients of the major inorganic ions are in near equilibrium with the energy of hydrolysis of ATP, the electric potential between the phases and each other (Masuda, T. et al, J.Biol.Chem. 265: 20321-20334, 1990). We have now shown that simple metabolic means may alter the energy of ATP hydrolysis (Kashiwaya, Y. et al, Am. J. Cardiol. 80: 50A-64A, 1997), and with that, restore the normal gradients of inorganic ions in patients with severe injuries or burns. By altering replacement therapy to include metabolic as well as simple volumetric considerations, one should be able to decrease both morbidity and mortality of acute hemorrhage and burns. These concepts will be the topic of a meeting to be held at the Academy of Medicine on Sept 16-18, 1998.