MECHANISMS GOVERNING SPINDLE ALIGNMENT IN C ELEGANS

线虫胚胎的最初几个分裂都有这两个例子 两个女儿都相同的增殖性分裂， 以及女儿们表现出不同的分化 命运。目前正在对该系统的动态特性进行研究 细胞骨架是分化分裂所特有的。在……里面 具体：发现因素被分离到一个区域 单元格；分割通常是不对称的，因为 有丝分裂器官中心体的不对称性；有丝分裂 可以看到设备在预先形成的极轴上对准。这个 该项目的目的是同时使用活体成像和相关 电子显微镜研究这些现象，以获得洞察力 关于细胞多样性产生的机制。这个 本研究的重点是阐明微管在血管内皮细胞中的作用。 将纺锤体定位在不对称细胞分裂的早期 秀丽隐杆线虫。我们将使用活体荧光显微镜 为了表征微管的组织和动力学 野生型和突变型胚胎的纺锤体排列。我们特别是 对表现出动态变化的突变体感兴趣 微管的组织以确定参与的基因 主轴对齐。此外，我们还将检查细胞的超微结构。 皮质微管附着部位。结果将提供洞察力 关于卵裂的基本机制和分子组成 决定性单元划分中的平面规范。SCXENTXFXC 子项目资助编号：P41RR00570-27

The first few divisions in C elegans embryo has examples of both proliferative divisions in which both the daughters are equivalent, and differentiative divisions in which the daughters take on different fates. Studies are being undertaken of the dynamic properties of the cytoskeleton that are unique to differentiative divisions. In particular: factors are found to be segregated to one region of the cell prior to division; divisions are usually asymmetrical due to an asymmetry in the centrosomes of the mitotic apparatus; the mitotic apparatus is seen to align on a preformed axis of polarity. The purpose of this project is to use both in vivo imaging and correlative electron microscopy to study these phenomenon so as to gain insights as to the mechanisms used in the generationof cell diversity. The focus of this study is to elucidate the role of microtubules in positioning the spindle in early asymmetric cell divisions of Caenorhabditis elegans. We will use in vivo fluorescence microscopy to characterize the organization and dynamics of microtubules during spindle alignment in wildtype and mutant embryos. We are especially interested in mutants that exhibit alterations in the dynamic organization of microtubules in order to identify genes involved in spindle alignment. In addition, we will examine the ultrastructure of cortical microtubule attachment sites. Results will provide insight into the basic mechanisms and molecular components underlying cleavage plane specification in determinative cell divisions. SCXENTXFXC SUBPROJECT GRANT NUMBER: P41RR00570-27