METABOLIC EFFECT OF TRP64ARG MUTATION IN BETA 3 ADRENERGIC RECEPTORS IN DIABETES

贝勒医学院和人类医学系的研究人员 公共卫生学院的遗传学，健康科学中心， 德克萨斯州休斯顿将研究β肾上腺素能受体的作用 西班牙裔男性和女性对脂和糖代谢的激活 患有非胰岛素依赖型糖尿病(NIDDM)。的目标是 这些研究是为了确定是否存在一个可预测的 β3-肾上腺素能受体突变与机体的关系 墨西哥裔美国人的脂肪分布与脂肪和葡萄糖代谢。 通过使用营养和锻炼作为实验操作，我们计划 为了确定这种突变在燃料调节中的作用 健康人群底物代谢及其在非胰岛素依赖型糖尿病中的失调科目 将在哈里斯县医院服务的患者中确定 东南部和南部的地区和斯塔尔县健康研究 德克萨斯州。它们将根据基因组DNA中的模式进行表征 从白细胞、身体成分和腹部脂肪分布来看 使用磁共振成像，将测量血浆葡萄糖和胰岛素水平 禁食期间和口服葡萄糖后。大约850 受试者将在横断面研究中确定特征，其中24人 将使用稳定同位素对这些主题进行更深入的研究 基线以下、喂食、禁食、休息和锻炼的技术 条件。24名受试者的这些研究将在一次过后重复。 以减肥为目标的渐进式减肥月 体重减轻5-10公斤，腹内脂肪减少5%-15%。

Investigators in Baylor College of Medicine and the Department of Human Genetics at the School of Public Health, Health Sciences Center, Houston, TX will study the effects of beta adrenergic receptor activation on lipid and glucose metabolism in Hispanic men and women with non-insulin-dependent diabetes mellitus (NIDDM). The objective of these studies is to determine whether there is a predictable relationship between the beta 3-adrenergic receptor (AR) mutation, body fat distribution and lipid and glucose metabolism in Mexican-Americans. By using nutrition and exercise as experimental manipulations, we plan to determine the role of this mutation in the regulation of fuel substrate metabolism in health and its dysregulation in NIDDM. Subjects will be identified among patients served in the Harris County Hospital District and the Starr County Health Studies in Southeast and South Texas. They will be characterized according to patterns in genomic DNA from leukocytes, body composition and distribution of abdominal fat using MRI, and plasma levels of glucose and insulin will be measured during fasting and after oral glucose administration. Approximately 850 subjects will be characterized in the cross-sectional study and 24 of these subjects will be studied more intensively using stable isotopic techniques under baseline, fed, fasting, resting, and exercising conditions. These studies in the 24 subjects will be repeated after one month of gradual weight reduction with the objective of decreasing body weight by 5 to 10 kg and reducing intraabdominal fat by 5% to 15%.