ACTION SPECTRUM OF DNA SYNTHESIS INHIBITION USING FEMTOSECOND ILLUMINATION

我们已经制作了一个光损伤的作用谱 通过监测从700到900 nm的飞秒照射 不加染料培养的HeLa细胞DNA合成抑制。 剂量(Int)定义为峰值辐照度在 样本乘以焦点体积的总驻留时间 手机。(辐照度i以光子为单位/S((m2，n为 单个激发事件所需的光子，或激发 秩序。)因为我们还没有确定它的激发顺序 在所有波长的损伤，此作用谱表示为n= 2、定义双光子事件。测量的损伤阈值为 比典型成像剂量高出数量级。

We have produced an action spectrum of photodamage using femtosecond illumination from 700 to 900 nm by monitoring the onset of DNA synthesis inhibition in cultured HeLa cells without added dyes. Doses (Int) are defined as the nth power of the peak irradiance at the sample multiplied by the total dwell time of the focal volume in a cell. (The irradiance, I, is in photons/s((m2, and n is the number of photons required for a single excitation event, or the excitation order.) Since we have not yet determined the excitation order of damage at all wavelengths, this action spectrum is expressed with n = 2, defining two-photon events. The measured damage thresholds are orders of magnitude above typical imaging doses.