PHOTOBLEACHING PROBES USING FLUORESCENCE CORRELATION SPECTROSCOPY: THEORETICAL

在单光子和多光子荧光显微镜中， 荧光标记物在强光下的降解是一个限制因素。 荧光团在强光照下会发生光漂白 通过单分子或双分子反应， 光子能量和环境起主要作用。 可靠 技术的光漂白量子产率（？B）测量值是 对于从根本上理解这些机制和途径至关重要 参与漂白动力学。 在单分子水平上， 荧光相关光谱（FCS）是一种强有力的技术 用于扩散以及三重态表征。 的理论 已经进行了分析，以扩展FCS的使用， 光漂白研究。 在这些分析中，三重态和第一激发态 单线态被认为是可能的光漂白途径。 环境效应，如单线态氧和外部重原子 的影响，也进行了讨论。

In one- and multi-photon fluorescence microscopy, photobleaching of the fluorescent markers under intense light is a limiting factor. A fluorophore under an intense illumination can undergo photobleaching through either unimolecular or bimolecular reaction in which the photon energy and the enviroment play a major rule. Reliable techniques for photobleaching quantum yield (?b) measurements are crucial for fundamental understanding of the mechanisms and pathways involved in bleaching dynamics. On a single molecule level, fluorescence correlation spectroscopy (FCS) is a powerfull technique for diffusion as well as triplet-state characterization. Theoritical analysis has been carried out to extend the use of FCS for photobleaching studies. In those analysis, triplet- and first excited singlet-state are considered as possible photobleaching pathways. Enviromental effects, such as singlet oxygen and external heavy-atom effects, were also discussed.