BIOTINYLATED ERYTHROCYTES IN PATIENTS WITH SICKLE CELL DISEASE

镰刀状红细胞会发生一些重要的细胞变化和 选择的压力。在这项研究中，我们通过以下方式验证了生物素红细胞标记 与标准的51CR标签进行比较，并用它来研究 随着年龄的增长发生在镰状细胞中。镰刀状红细胞的寿命要短得多 但这两种标记给出了相同的结果 单元类型。数量可变的镰刀状红细胞消失，但不是正常的 回输后最初几个小时的血液循环。数字 生物素化镰状网织红细胞的数量减少了50% 24小时和48小时后为75%，并逐渐减少 每个细胞的网织数。标记的镰状细胞密度较大。 在回输后的头四到六天内增加，较小 此后发生了变化。一小群非常轻的，标记为镰刀状的红细胞 在最初的几天后，数量基本上保持不变。HBF含量 在回输后分离的生物素化镰刀状红细胞中检测到， 从而可以估计F细胞和非F细胞的寿命。这个 缺乏HBF的镰刀状B-RBC的寿命估计约为两周， 而F细胞存活6-8周。

SIckle RBC are subject to a number of important cellular changes and selection pressures. In this study, we validated a biotin RBC label by comparison to the standard 51CR label, and used it to study changes that occur in sickle cells as they age. Sickle RBC had a much shorter lifespan than did normal RBC, but the two labels gave equivalent results for both cell types. A variable number of sickle, but not normal, RBC disappeared the circulation during the first few hours after reinfusion. The number of biotinylated sickle reticulocytes was decreased by fifty percent after 24 hours and 75% after 48 hours, with a gradual decrease in the amount of reticulum per cell. The labeled sickle cells exhibited major density increases during the first four to six days after reinfusion, with smaller changes thereafter. A small population of very light, labeled sickle RBC was essentially constant in number after the first few days. HbF content was determined in isolated biotinylated sickle RBC after reinfusion, allowing an estimate of lifespan for F cells and non-F cells. The lifespan of sickle B-RBC lacking HbF was estimated to be about two weeks, whereas F cells survived six to eight weeks.