GENETIC ANALYSES OF EBV'S IMMORTALIZING GENES

描述(改编自申请者摘要)：遗传分析 病毒基因通过以下途径促进人类B淋巴细胞永生化 爱泼斯坦-巴尔病毒(Epstein-Barr Virus，EBV)由于缺乏可靠的手段而受到阻碍 用于在没有污染野生型病毒的情况下测试突变病毒。 萨格登博士与他的长期同事苏格登博士合作。 Hammerschmidt已经开发出EBV的衍生品，称为迷你EBV，它可以 在大肠杆菌中以质粒的形式繁殖，包装在人类细胞系中 含有EBV辅助病毒，并能够单独永垂不朽 B淋巴细胞。定义的突变可以被引入到迷你EBV中 大肠埃希菌宿主。Sugden博士和Hammerschmidt博士建议测量 选定的EBV永生化基因的明确突变对效率的影响 永垂不朽。这些基因也将被分析，以确定 它们是启动和/或维护 永垂不朽。最后，迷你EBV包含最少量的 将构建永生所需的遗传信息。

DESCRIPTION (Adapted from Applicant's Abstract): Genetic analysis of the viral genes that contribute to the immortalization of human B-lymphocytes by Epstein-Barr virus (EBV) has been hampered by the lack of a reliable means for testing mutant viruses in the absence of contaminating wild-type virus. Dr. Sugden, in collaboration with his longstanding colleague, Dr. Hammerschmidt have developed derivatives of EBV, termed mini-EBVs, that can be propagated as plasmids in E. coli, packaged in a human cell line containing an EBV helper virus, and are capable alone of immortalizing B-lymphocytes. Defined mutations can be introduced into mini-EBVs in their E. coli host. Drs.Sugden and Hammerschmidt propose to measure the effects of defined mutations in selected EBV immortalizing genes on the efficiency of immortalization. These genes will also be analyzed to determine whether they are required for the initiation and/or the maintenance of immortalization. Finally, mini EBVs containing the minimal amount of genetic information required for immortalization will be constructed.