GENETIC CONTROL OF MUTATION IN DROSOPHILA

工作总结：本研究旨在探讨果蝇DNA修复、染色体结构和突变之间的关系。增加突变频率的突变（突变体）已在mu2基因中鉴定并表征。这些突变似乎破坏了卵母细胞中的染色质结构，但不是精子染色体，因此卵母细胞染色体不能正确修复，而是被一个新的端粒覆盖。鉴于mu2突变也增加有丝分裂重组，因此可以合理地预期MU2蛋白也与体细胞染色体相关。已经制备了MU2蛋白的抗体，并将用于确定细胞内定位。我们还计划利用酵母双杂交系统寻找可能与MU2相互作用的其他基因产物。还将使用mu2的突变和HP 1（推定的加帽蛋白）和Ku70的突变来制备双突变体。- 染色质，DNA损伤，DNA修复，果蝇，异染色质，卵母细胞，端粒

Summary of Work: This project is designed to determine the relationships among DNA repair, chromosome structure, and mutagenesis in Drosophila melanogaster. Mutations that increase the mutant frequency (mutators) have been identified in the mu2 gene and characterized. These mutations appear to disrupt chromatin structure in the oocyte, but not the sperm chromosomes, so that oocyte chromosomes are not repaired properly, and instead are capped with a new telomere. Given that mu2 mutations also increase mitotic recombination, it is reasonable to expect that the MU2 protein also associates with somatic chromosomes. Antibodies have been made to the MU2 protein and will be used to ascertain intracellular localization. We also plan to look for other gene products that might interact with MU2 using the yeast two- hybrid system. Double mutants will also be made using mutations for mu2 and mutations for either HP1, a putative capping protein, and Ku70. - Chromatin, DNA Damage, DNA Repair, Drosophila Melanogaster, Heterochromatin, Oocyte, Telomere