SUBSTANCE P-SPECIFIC CONDUCTING POLYMER ELECTRODE

描述：神经活性肽是多种人类免疫系统中的关键参与者。 对疼痛、应激、出血、输血、体位和缺氧的反应，以及 影响免疫系统。 身体的某个系统可以为我们提供关于 健康或疾病状态。目前还没有特异性神经肽 电极可用。 我们建议开发一种用于特异性传感P物质的电极， 一种参与中枢内源性疼痛控制的神经肽 系统我们将利用一种新的电化学配准方法 的电导率变化，发生在专门设计的导电 聚合物基质上固定的协同相互作用 抗体与选定的神经肽。这种电极的特异性可以是 通过选择固定化抗体的特异性来设计。第一阶段将是 致力于开发和校准P物质专用电极 体外第一阶段将致力于电极的小型化， 体内应用。具有设计特异性的这种电极阵列将 能够从同一部位检测到几种肽神经递质。 拟定商业应用： 基于我们的方法开发的电极将代表一个重大的进步 在检测肽神经递质，并将是一个更好的替代品， 目前使用的免疫分析法。 使用这种电极的阵列 由于特异性不同，将有可能检测几种肽类神经递质 在体内和从同一网站，这将允许研究的相互作用， 神经内分泌系统的神经肽。

DESCRIPTION: Neuroactive peptides are the key players in a variety of human responses to pain, stress, hemorrhage, transfusion, orthostasis and anoxia, and are affecting the immune system The concentration of a specific neuropeptide in a certain system of the body can provide us with critical information on the state of health or disease. At present, there are no neuropeptide-specific electrodes available. We propose to develop an electrode for specific sensing of Substance P, a neuropeptide involved in the endogenous pain control in the central nervous system. We will be utilizing a novel approach of electrochemical registration of the conductivity changes, occurring in specifically designed conductive polymer matrix upon cooperative interaction of the immobilized on the matrix antibodies with selected neuropeptide. Specificity of such electrode can be designed by choosing the specificity of immobilized antibody. Phase I will be dedicated to the development and calibration of Substance P specific electrode in vitro. Phase I will be dedicated to miniaturization of the electrode and in vivo applications. An array of such electrodes with designed specificity will be able to detect several peptide neurotransmitters from the same site. PROPOSED COMMERCIAL APPLICATION: Electrodes, developed based on our approach, will represent a significant advance in the detection of peptide neurotransmitters, and will be a far better alternative to the immunoassays, which are currently used. Using an array of such electrodes with different specificity, it will be possible to detect several peptide neurotransmitters in vivo and from the same site, which will allow to study the interactive actions of neuropeptides on the neuroendocrine system.