CORE--NEUROPATHOLOGY

神经病理学核心（NP核心）的目的是进行尸检 爱因斯坦老龄化项目参与者的大脑协议 使用最先进的神经病理学方法的研究（EAS）。的功能 收集、储存和分发死后材料， 数据如下： 将活动与病理科随叫随到服务相结合， 入组EAS的受试者的CNS尸检可以在 及时的方式和根据协议。 根据方案进行神经病理学评价，并收集 使用以下标准化所有大脑的神经病理学数据 方法：a.大体检查，B。苏木精和伊红染色切片， C.硫磺素-S荧光显微镜; PHF-tau免疫染色 用于阿尔茨海默病病理分期的切片 对于血管病变的病例，两名神经病理学家独立地 评价大体和显微镜数据。临床病理学共识 诊断已经达到。 在那些神经元丢失或路易体丢失的病例中， 黑质，多个切片用 tau和泛素的抗体。路易体的密度以及 CA 2/3区Lewy型神经突的存在和严重程度 海马是决定性的。 非阿尔茨海默变性痴呆的特征在于 免疫细胞化学和神经丝抗体（SMI-31），泛素 和PHF-tau（PHF-1和TG 3）。 NP Core为项目3提供固定和冷冻的大脑样本， 免疫细胞化学、图像分析和酶联免疫吸附 测定。组织不时提供给其他研究者， 所请求 NP Core确定来自脑组织的载脂蛋白E基因型， 临床中心采集的血液样本。 组织样本被储存起来用于未来的研究和神经病理学研究 为可能与该计划有关的任何试点项目执行 项目 所有数据都使用标准化的 协议和程序。

The purpose of the Neuropathology Core (NP Core) is to perform postmortem protocols on brains of participants in the projects of the Einstein Aging Study (EAS) using state-of-the-art neuropathologic methods. The functions of the NP Core are to collect, bank and distribute postmortem material and data as follows: Integrate activities with the Pathology Departmental on-call service so that CNS autopsies of subjects enrolled in the EAS can be performed in a timely fashion and according to protocol. Perform the neuropathologic evaluations according to protocol and collect standardized neuropathologic data from all brains using the following methods: a. Gross examination, b. Hematoxylin and eosin stained sections, c. Thioflavin-S fluorescent microscopy, and d. PHF-tau immunostained sections for staging Alzheimer type pathology. For cases with vascular lesions, the two neuropathologists independently evaluate gross and microscopic data. A consensus clinicopathological diagnosis is reached. In those cases with either neuronal loses loss or Lewy bodies in the substantia nigra, multiple sections are double immunostained with antibodies to tau and ubiquitin. The density of Lewy bodies as well as the presence and severity of Lewy-types neurites in CA2/3 region of hippocampus are determined. Non-Alzheimer degenerative dementia are characterized with immunocytochemistry and antibodies to neurofilament (SMI-31), ubiquitin and PHF-tau (PHF-1 and TG3). The NP Core provides fixed and frozen brain samples to Project 3 for immunocytochemical, image analytical and enzyme linked immunosorbent assays. Tissue is provided to other investigators from time to time, as requested. The NP Core determines apolipoprotein-E genotypes from brain tissue and on blood samples collected by the Clinical Core. Tissue samples are banked for future studies and neuropathologic studies are performed for any pilot project that may related to the program project. All data is communicated to the Statistical Core, using standardized protocols and procedures.