CORE--NEUROPATHOLOGY

神经病理学核心提供完整的脑库和神经病理学 匹兹堡ADRC的诊断服务。保证…的银行业务 质量上乘的组织，尸检时间短，尸检弧形 24小时执行。当时的大脑呈矢状等分。 验尸报告。右半部分被解剖并分发给ADRC 调查人员和被闪存冻结的银行业务。大脑的左半部是 已修复并可用于磁共振成像。定影后 为进行神经病理学诊断，对左半脑进行了广泛的取样 检测包括H&E、刚果红、免疫细胞化学和银染。 对左半脑的显微分析是根据 修正经修订的CERAD议定书。除 神经退行性疾病的鉴别诊断，那些大脑 发现有阿尔茨海默病改变的人是根据 公布的缠结密度标准。脑库被编目了， 与神经病理诊断相关，以便更好地利用 向ADRC和其他调查人员描述了组织的特征。神经病理学 诊断结果将在一个月内提供给ADRC数据库和家庭 对死亡的恐惧。截至1993年，神经病理核心银行约有50家ADRC 每年的验尸报告。此外，100名控制组大脑将被储存并 以常规的大体和显微神经病理为特征 考试。核心维护一个参考实验室(包括Ar/Kr 激光共聚焦显微镜和工作站)，用于开发详细和 定量分析。

The Neuropathology core provides complete brain banking and neuropathology diagnostic services for the Pittsburgh ADRC. To assure banking of excellent quality tissues with short post-mortem times, autopsies arc performed on a 24 hour basis. The brain is bisected sagittally at the time of autopsy. The right half is dissected for distribution to ADRC investigators and flash-frozen banking. The left half of the brain is fixed and made available for magnetic resonance imaging. After fixation the left hemi-brain is extensively sampled for neuropathology diagnostic tests including H&E, Congo Red, immunocytochemical and silver stains. Microscopic analysis of the left hemi-brain is performed according to a modification of the revised CERAD protocol. In addition to the differential diagnostics for neurodegenerative diseases, those brains found to possess changes of Alzheimer's Disease are staged according to published criteria of tangle densities. The brain bank is catalogued and correlated with neuropathology diagnoses to make available well characterized tissues to ADRC and other investigators. Neuropathology diagnoses are supplied to the ADRC database and families within one month of death. As of 1993 the neuropathology core banks approximately 50 ADRC autopsies per year. Additionally 100 control brains will be banked and characterized by routine gross and microscopic neuropathologic examination. The core maintains a reference laboratory (including a Ar/Kr laser confocal microscope and workstation) for development of detailed and quantitative analyses.