CORE--CELL SCIENCE FACILITY

大量与糖尿病相关的研究调查在 马萨诸塞大学医学中心依赖于细胞的使用 培养和相关技术。细胞科学核心(CSC)设施 通过提供四个主要方面的服务来满足调查人员的需求 领域：1)各种常规细胞培养服务，2)DERC细胞 已经建立了银行，为参与者提供各种细胞 为他们的工作行，3)一些专门的免疫细胞培养 服务，包括淋巴细胞分离和刺激，以及 生产单抗，以及4)细胞分选在我们的FACS 配备FACS440、FACSCAN和双激光FACSTAR的设施 再加。CSC位于改建并配备了 跨部门组织培养实验室综合体，由 医学中心，与糖尿病相关的研究代表了大约 70%的活动。设施包括用于细胞培养的单独房间， 每一个都配备了二氧化碳组织培养孵化器和层流 通风帽。中心实验室区域包含一个程序化细胞 冷冻机、离心机、用于细胞存储的液氮冰箱，以及 制备培养基用的设备。荧光激活细胞 分拣机位于一个单独的专门建造的房间里。组织 培养服务包括培养准备和无菌检测， 原代培养物的制备、维护和分发 连续细胞系和细胞的冷冻保存。协助 单抗生产包括杂交克隆的制备，以及 向个别调查人员提供上清液 他们可以筛查特定抗体的产生。FACS设施是 用于细胞的定量分析和分离。这个核心 通过以下方式提高糖尿病研究的生产力：a)提供服务 不在个别实验室提供；b)允许调查人员 专注于他们的特定项目，而无需开发细胞 银行、细胞分选能力和其他设施 实验室；以及c)鼓励调查人员之间的互动 共享这一核心设施的资源。

A large number of diabetes-related research investigations at the University of Massachusetts Medical Center depend upon the use of cell culture and associated techniques. The Cell Science Core (CSC) Facility satisfies the needs of investigators by providing services in four major areas: 1) a variety of routine cell culture services, 2) a DERC cell bank has been established to provide participants with a variety of cell lines for their work, 3) a number of specialized immune cell culture services, including lymphocytes separation and stimulation, and the production of monoclonal antibodies, and 4) cell sorting in our FACS Facility equipped with an FACS 440, FACSCAN and a Dual Laser FACSTAR Plus. The CSC is located within the reconstructed and equipped interdepartmental tissue culture laboratory complex provided by the Medical Center, and diabetes-related research represents approximately 70% of its activity. Facilities include separate rooms for cell culture, each of which is equipped with CO2 tissue culture incubators and laminar flow hoods. A central laboratory area contains a programmed cell freezer, centrifuges, liquid nitrogen refrigerators for cell storage, and equipment for preparation of culture media. Fluorescence activated cell sorters are located in a separate specially constructed room. Tissue culture services include media preparation and sterility testing, preparation of primary cultures, maintenance and distribution of continuous cell lines, and cryopreservation of cells. Assistance in monoclonal antibody production includes preparation of hybrid clones, and provision to individual investigators of supernatant culture fluids which they can screen for specific antibody production. The FACS facility is used for quantitatively analyzing and separating cells. This core enhances the productivity of diabetes research by: a) providing services not available in individual laboratories; b) allowing investigators to focus on their particular projects without the need to develop cell banks, cell sorting capabilities and other facilities in their own laboratories; and c) by encouraging interactions between investigators who share the resources of this core facility.