CORE--CELL SCIENCE FACILITY

大量与糖尿病相关的研究调查， 马萨诸塞州大学医学中心依靠细胞的使用 文化及相关技术。 Cell Science Core（CSC） 满足调查人员的需求，提供四个主要服务， 领域：1）各种常规细胞培养服务，2）DERC细胞 建立了一个细胞库，为参加者提供各种细胞 他们的工作线，3）一些专门的免疫细胞培养 服务，包括淋巴细胞分离和刺激， 单克隆抗体的生产，以及4）我们的FACS中的细胞分选 配备FACS 440、FACSCAN和双激光FACSTAR的设施 Plus. CSC位于重建和装备的 提供的跨部门组织培养实验室综合大楼 医学中心和糖尿病相关研究约占 70%的活动。 设施包括单独的细胞培养室， 每一个都配备了二氧化碳组织培养箱和层流 气流罩 中心实验室区域包含一个编程细胞 冷冻机、离心机、用于细胞储存的液氮冰箱，以及 用于制备培养基的设备。 荧光激活细胞 分拣机位于一个单独的专门建造的房间内。 组织 培养服务包括培养基制备和无菌测试， 原代培养物的制备、维护和分配 连续细胞系和细胞的冷冻保存。 协助 单克隆抗体生产包括制备杂交克隆，和 向个体研究者提供上清液， 他们可以筛选特异性抗体的产生。 FACS设施是 用于细胞的定量分析和分离。 这一核心 通过以下方式提高糖尿病研究的生产力：a）提供服务 单个实验室无法获得; B）允许研究者 专注于他们的特定项目，而不需要开发细胞 细胞库、细胞分选能力和其他设施 实验室;以及c）鼓励研究人员之间的互动 共享这个核心设施的资源

A large number of diabetes-related research investigations at the University of Massachusetts Medical Center depend upon the use of cell culture and associated techniques. The Cell Science Core (CSC) Facility satisfies the needs of investigators by providing services in four major areas: 1) a variety of routine cell culture services, 2) a DERC cell bank has been established to provide participants with a variety of cell lines for their work, 3) a number of specialized immune cell culture services, including lymphocytes separation and stimulation, and the production of monoclonal antibodies, and 4) cell sorting in our FACS Facility equipped with an FACS 440, FACSCAN and a Dual Laser FACSTAR Plus. The CSC is located within the reconstructed and equipped interdepartmental tissue culture laboratory complex provided by the Medical Center, and diabetes-related research represents approximately 70% of its activity. Facilities include separate rooms for cell culture, each of which is equipped with CO2 tissue culture incubators and laminar flow hoods. A central laboratory area contains a programmed cell freezer, centrifuges, liquid nitrogen refrigerators for cell storage, and equipment for preparation of culture media. Fluorescence activated cell sorters are located in a separate specially constructed room. Tissue culture services include media preparation and sterility testing, preparation of primary cultures, maintenance and distribution of continuous cell lines, and cryopreservation of cells. Assistance in monoclonal antibody production includes preparation of hybrid clones, and provision to individual investigators of supernatant culture fluids which they can screen for specific antibody production. The FACS facility is used for quantitatively analyzing and separating cells. This core enhances the productivity of diabetes research by: a) providing services not available in individual laboratories; b) allowing investigators to focus on their particular projects without the need to develop cell banks, cell sorting capabilities and other facilities in their own laboratories; and c) by encouraging interactions between investigators who share the resources of this core facility.