GENETIC ASSOCIATIONS OF CANNABINOID RECEPTOR ALLELES IN DRUG DEPENDEN

据报道，脑部大麻受体基因启动子区域的PolyA区相关的特定等位基因类型与静脉注射阿片类药物、可卡因和/或强效可卡因的使用有关，但与酒精依赖无关。这个项目的目标是使用我们在其他候选基因站点研究的药物和酒精依赖对象的集合来提供对这份报告的独立检查。我们还将扩大这项研究的范围，以检查大麻受体基因启动子上的等位基因与对大麻的初级药物依赖之间的关联。在过去的几个月里，我们已经招募了9名仅依赖大麻的受试者，最近已经开始分析我们储存的450名药物和酒精依赖受试者的DNA的这个等位基因。到目前为止，我们的样本中还没有关于研究中的大麻素标志物和物质依赖亚型之间存在关联的可报告结果。在明年，我们预计再招募40-50名大麻依赖受试者，并为这些受试者和我们之前确定的药物和酒精依赖受试者完成大麻类等位基因的初步基因分型。

Specific allele types associated with poly-A tract in the promoter region of the brain cannabinoid receptor gene have been reported to be associated with intravenous opiate and cocaine and/or crack cocaine use but not with alcohol dependence. The goal of this project is to provide an independent examination of this report using a collection of drug and alcohol dependent subjects we have been studying at other candidate genetic sites. We will also extend the study to include examination of associations between alleles at the cannabinoid receptor gene promoter and primary drug dependence on cannibis. We have enrolled 9 cannabis-dependent only subjects during the last few months and have recently begun to analyze the DNA from our banked samples of 450 drug and alcohol dependent subjects at this allele. There are no reportable results to date from our sample as to the presence of associations at the cannabinoid marker under study and subtypes of substance dependence. During the next year we anticipate recruiting 40-50 additional cannabis dependent subjects and to complete the initial genotyping at the cannabinoid allele for these and our previously identified cohorts of drug and alcohol dependent subjects.