CORE--ANIMAL

动物核心的目的是为研究者提供 为设计提供科学、技术和资金支持 和执行他们的研究。一个集中的国家的可用性 艺术设施是需要的收购和住房的菲舍尔 将344只大鼠用于缺血再灌注和抗氧化 干预研究，即TNF在衰老和缺血中的表达- 再灌注心脏损伤的复合物III，氧化修饰的 Rieske铁蛋白、心磷脂状态和游离脂肪酸氧化， 巯基防御机制此外，该设施将需要 对病原体进行现场短期和长期干预研究- 自由的环境和发展的一种新的模式，加速 衰老 该核心的具体目标是1。协调收购和 Fischer 344大鼠及其组织在计划项目中的分布 参与缺血再灌注研究的研究者，2.提供 Fischer 344大鼠和其他参与这些研究的啮齿类动物的饲养所 在一个集中的专门设施下， 符合适用于老年学研究的健康标准 （无病原体环境、体重测量、身体和 组织学检查），3.为科学设计提供帮助 和执行涉及特殊饮食和药理学的方案 研究衰老动物的治疗，以及4.为新员工提供培训 微隔离器应用程序项目的研究人员 与老年学研究和研究有关的特殊技术 涉及无病原体的动物污渍。 为项目研究者提供的服务将由 一个由核心主任（V. Monnier）组成的团队，由2名副主任协助， 董事，即动物科学和屏障设施专家， NIA啮齿动物（Dr. Sell，PhD）和兽医（N. Kleinman，DVM）。50%全职研究助理将承担日常工作 与动物的工作人员合作运营设施 在CWRU的资源中心。 预计这种广泛设计的动物核心将使我们能够 整合与计划项目相关的新项目， 发挥其主要作用。

The objective of the Animal Core is to provide investigators of the Program Project scientific, technical and financial support for the design and execution of their studies. The availability of a centralized state of the art facility is needed for the acquisition and housing of the Fischer 344 rats that will be utilized in the ischemia-reperfusion and antioxidant intervention studies, i.e. TNF expression in the aging and ischemia- reperfused heart damage to complex III, oxidative modification of the Rieske iron protein, cardiolipin status and free fatty acid oxidation and the thiol defense mechanism. In addition, the facility will be needed to conduct on-site short and longer term intervention studies in a pathogen- free environment and the development of a novel model of accelerated aging. The specific aims of this core are 1. To coordinate the acquisition and distribution of Fischer 344 rats and their tissues among Program Project investigators involved in ischemia-reperfusion studies, 2. To provide housing for Fischer 344 rats and other rodents involved in these studies under a centralized, specialized facility routinely monitored for compliance with standards of health applicable to gerontological studies (pathogen free environment, body weight measurement, physical and histological examination), 3. To provide assistance with scientific design and execution of protocols involving special diets and pharmacological treatments to study aging animals, and 4. To provide training for new investigators of the Program Project in the utilization of micro-isolators and special techniques pertinent to gerontological studies and studies involving pathogen-free animal stains. Services to the Investigators of the Program Project will be provided by a team consisting of the Core director (V. Monnier) assisted by 2 Co- directors, i.e. an expert in Animal Sciences and Barrier Facility for longevity studies with NIA rodents (DR. Sell, PhD) and a Veterinarian (N. Kleinman, DVM). A 50% FTE Research Assistant will assume the day-to-day operation of the facility in collaboration with the staff of the Animal Resources Center at CWRU. It is expected that this broadly designed Animal Core will allow us to integrate new projects of relevance to the Program Project while fulfilling its primary role.