CORE--TISSUE CULTURE, REAGENTS, AND ELISA

核心B将在计划项目中扮演两个主要角色。核心B将是 负责维护和供应共享的细胞系/基因 构建、融合蛋白、杆状病毒构建体和抗体 参与该计划项目的所有调查人员。第二，核心意志 执行Abeta酶联免疫吸附试验以量化总Abeta、Abeta42(和其他 以及时和方便的方式为所有人提供 参与该计划项目的调查人员。核心B基本上是 旨在用作中央存储库，用于维护和提供 在过去的几年里产生了大量非常有用的试剂 在过去的十年里，参与该项目的调查人员进行了调查。这些 包括大量稳定转导的哺乳动物的试剂 细胞系和相应的转基因cdna构建物，几种融合 蛋白质/杆状病毒构建物和相应的细胞系以及广泛的 大量收集特异性多克隆和单抗 促进持续协作和整体实验效率 在计划项目中。因为FAD的一个主要分子后果 早老素基因突变作为一种增加的比例 Abeta42：Abeta40，所有四个项目和转基因核心将需要 获取定量测量的有效和方便的手段 阿贝塔。因此，核心B将可用于进行Abeta酶联免疫吸附试验 只要项目调查员需要，就可以随时提交。虽然这笔费用 维护核心试剂并执行Abeta酶联免疫吸附试验(和其他 将产生ELISA，例如，在有可用的情况下，用于早产儿) 按核心B，个别项目支出，包括大型项目支出- 用于特定生物实验或蛋白质的细胞系的构建 净化将由个别项目预算支付。核心B 活动将由坦齐博士和塞尔科博士在日常工作中监督 中心的运作将由威尔玛·瓦斯科博士监督。

Core B will have two primary roles in the Program Project. Core B will be responsible for maintaining and supplying shared cell lines/cDNA constructs, fusion proteins, baculovirus constructs, and antibodies for all investigators involved in the Program Project. Secondly, the Core will perform the Abeta ELISA for quantitating total Abeta, Abeta42 (and other ELISA's as they are developed), in a timely and convenient manner for all investigators involved in the Program Project. Core B is basically intended to serve as a central repository for maintaining and supplying a large number of highly useful reagents that have been generated over the last decade by the Investigators involved in the Program Project. These reagents which include a large variety of stably-transfected mammalian cell lines and corresponding transgene cDNA constructs, several fusion proteins/baculovirus constructs and corresponding cell lines and extensive collection of specific polyclonal and monoclonal antibodies greatly facilitate ongoing collaborations and overall experimental efficiency within the Program Project. Since a major molecular consequence of FAD mutations in the presenilin genes as an increase in the ratio of Abeta42:Abeta40, all four projects and the transgenic core will require an effective and convenient means for obtaining quantitative measurements of Abeta. Therefore, Core B will be available to carry out the Abeta ELISA whenever desired by a Program Investigator. While the expenses for maintain the Core reagents and performing the Abeta ELISA (and other ELISAs, e.g. for presenilins, as they become available) will be incurred by Core B, individual project expenses including those for large scale- workup of cell lines for specific biological experiments or protein purifications will be covered by individual project budgets. Core B activities will be overseen by Dr. Tanzi and Dr. Selkoe while day-to-day operations of the Core will be supervised by Dr. Wilma Wasco.