FISH ASSAY FOR THE DETECTION OF T (14;18) (Q32;21)

描述(申请人摘要)：大约60%的B细胞 淋巴瘤的特征是存在t(14；18)(q32；q21)。 染色体易位。诊断和治疗后的随访目前 通过核型、Southern blotting或聚合酶链式反应进行，这些方法存在问题 特异性和敏感性。荧光原位杂交 解决了这些限制；然而，没有经过临床测试的FISH探针 可用于检测t(14；18)易位。第一阶段研究进展顺利 用于t(14；18)易位间期FISH分析的新型探针。vbl.使用 无论是正常样本还是肿瘤样本，探针的灵敏度和特异性都很高 都观察到了。使用来自多种组织类型的肿瘤标本，II期 研究将验证探针用于诊断的有效性。第二阶段研究将 还验证了它们在监测微小残留病方面的用途。在第二阶段 研究表明，探针也将进行优化，以减少背景杂交 信号。 建议的商业应用：不可用

DESCRIPTION (applicant's abstract): Approximately 60 percent of B-cell lymphomas are characterized by the presence of the t(14;18) (q32;q21) chromosomal translocation. Diagnosis and post-treatment follow-up are currently performed by karyotype, Southern blotting, or PCR, which have problems with specificity and sensitivity. Fluorescence in situ hybridization (FISH) addresses these limitations; however, no clinically tested FISH probes are available for detecting the t(14; 18) translocation. Phase I research developed novel probes for interphase FISH analysis of the t(14; 18) translocation. Using both normal and tumor samples, high sensitivity and specificity of the probes were observed. Using tumor specimens from multiple tissue types, Phase II research will validate use of the probes for diagnosis. Phase II research will also validate their use for monitoring minimal residual disease. In Phase II research, the probes will also be optimized to reduce background hybridization signal. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE