Ephrin A receptor tyrosine kinases in preventing axonal regeneration

本研究的目的是探讨EphA受体蛋白酪氨酸激酶(RPTK)及其配体在脊髓损伤(SCI)后不允许轴突再生的环境中的作用。分子生物学、蛋白质生物化学、神经成像追踪策略和行为分析将与T10水平的损伤大鼠结合使用，以分析这些蛋白质的表达。第一个目标将集中在脊髓损伤后中枢神经系统(CNS)在细胞核和氨基酸水平上的Eph A和ePhin A、mRNAs和蛋白质的时空表达。在第二个目标中，我们将确定Eph A RPTK和ephins作为排斥分子信号在脊髓损伤后限制轴突再生和功能恢复的可能作用。在本研究中，表达Eph A RPTKs或Ephins A氨基末端的融合蛋白将是单个Eph A RPTKs和ephins在再生纤维和局部环境中的时空表达，可能导致促进脊髓损伤后再生和功能恢复的新的治疗策略。

The goal of this study is to investigate the role of Eph A receptor protein tyrosine kinases (RPTK) and their ligands (ephrins) in producing a non- permissive environment for axonal regeneration after spinal cord injury (SCI). Molecular biology, protein biochemistry, neuroimaging tracing strategies and behavioral assays will be used in conjunction with injured rats at the T10 level to analyze the expression of these proteins. The first aim will focus on the spatial and temporal expression of Eph A and ephrin A, mRNAs and proteins, after spinal after Central Nervous System (CNS) lesion at the nuclei and amino acid level. The expression pattern will moderate spinal cord contusion, In the second aim, we will determine the possible role of Eph A RPTK and ephrins as repulsive molecular cues that restrict axonal regeneration and functional recovery after SCI. For this study, fusion proteins expressing the amino terminus of the Eph A RPTKs or ephrins A will be temporal and spatial expression of individual Eph A RPTKs and ephrins, both on the regenerating fibers and in the local environment, may lead to novel therapeutic strategies to enhanced regeneration and functional recovery after SCI.