CENTER FOR GENOME RESEARCH

在NHGRI的排序试点项目下，怀特黑德/麻省理工学院 基因组研究已经开发出一种可扩展和高效的过程 基因组测序。 这项建议的具体目的是将我们的试点项目转变为 生产中心由以下人员提供： (I)将行车线容量增加至每年2000万车道，方法是扩大 用于样品准备和购买毛细管车队的自动化系统 测序器；以及 (Ii)实现成品序列的生产率至少为750 每年10美分/基数，包括间接费用。 我们还致力于与NHGRI和其他中心密切合作 适应瞬息万变的环境，确保全面快速 人类基因组计划的成功。

Under NHGRI's sequencing pilot project, the Whitehead/MIT Center for Genome Research has developed a scalable and efficient process for genomic sequencing. The specific aim of this proposal is to convert our pilot project to a production center by: (i) increasing lane capacity to 20 M lanes per year, by expanding our automated systems for sample prep and purchasing a fleet of capillary sequencers; and (ii) achieving a production rate of finished sequence of at least 750 Mb per year at a cost of 10 cents/base including overhead. We are also committed to working closely with NHGRI and other centers to adapt to rapidly changing circumstances, to ensure the overall rapid success of the Human Genome Project.