FROM GENOMIC SEQUENCES TO PROTEIN STRUCTURE/FUNCTION

本计划项目申请的目标是分配结构和 对基因组开放阅读框的蛋白质产物的作用 没有已知的三维结构的同源物和 功能。与目前的做法不同，申请人和他的 项目负责人提议开展一项全面、协调的努力， 使用高通量结晶学方法测定蛋白质 从原子级结构起作用。精选的互补DNA 流感嗜血杆菌基因和其他指示的微生物将 克隆后在大肠杆菌中表达及纯化 蛋白质产品，以获得足够数量的蛋白质 结晶学、X射线结晶学和核磁共振三维结构 决定。

The goal of this Program Project application is to assign structure and function to the protein products of genomic open reading frames for which there are no homologues with known three dimensional structure and function. In a departure from current practice, the applicant and his project leaders are proposing to mount a comprehensive, concerted effort, using high throughput crystallographic approaches, to determine protein function from atomic level structure. Complementary DNA from selected Hemophilus influenzae genes, and other microorganisms as indicated, will be cloned for expression in Escherichia coli and purification of the protein products in order to obtain sufficient quantities of protein for crystallization and X-ray crystallographic and NMR 3D structure determinations.