Structural Informatics of alternative splicing

选择性剪接的结构信息学

• 批准号: 6689849
• 负责人: JOHN MOULT
• 金额: $ 17.08万
• 依托单位: UNIVERSITY OF MD BIOTECHNOLOGY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6689849
• 关键词: Internet; chemical models; computer data analysis; conformation; information systems; ligands; protein sequence; protein structure function; structural biology

This component of the program project provides informatics support and analysis. In the next phase, focusing on the structural impact of alternative splicing, there are five principle goals: First, development of a database that integrates a wide range of information relevant to alternative splicing in higher eukaryotes, particularly human, so that the most interesting and rewarding cases can be selected for experimental study. The design of the database is based on our experience with integrating information for target choice in the present phase of the project. Second, development of tools for automatically assessing the reliability of data indicating alternative splicing. At this stage of the development of the field, it is critical to be able to easily assess the data supporting a splice, before committing experimental effort. Third, production of structure models, allowing a visual appreciation of the likely impact of splicing on structure. Fourth, development of a comprehensive public database that will provide information on alternate splicing in a structural context, obtained from our own and work that of others, and using both models and experimental structures. This resource will allow users to obtain information about specific proteins, and to perform analyses on the general relationship between splicing, structure, function and disease. Fifth, the development and application of tools to analyze the relationship between splicing, structure and function. Tools for the analysis of function developed in the present phase will form the basis of these.

该计划项目的这一组成部分提供信息学支持和分析。在下一阶段，重点是选择性剪接的结构影响，有五个主要目标：第一，开发一个数据库，整合了广泛的信息，在高等真核生物，特别是人类的选择性剪接，使最有趣和最有价值的情况下，可以选择实验研究。数据库的设计是根据我们在项目现阶段为目标选择整合信息的经验。第二，开发自动评估数据可靠性的工具， 选择性剪接在油田开发的这个阶段，在进行实验工作之前，能够容易地评估支持拼接的数据是至关重要的。第三，制作结构模型，允许视觉欣赏拼接对结构的可能影响。第四，开发一个全面的公共数据库，提供结构背景下的选择性剪接信息，从我们自己的和其他人的工作中获得，并使用模型和实验结构。该资源将允许用户获得有关特定蛋白质的信息，并对剪接，结构，功能和疾病之间的一般关系进行分析。第五，开发和应用工具，分析拼接、结构和功能之间的关系。本阶段开发的功能分析工具将构成这些工具的基础。