Structural Informatics of alternative splicing

该方案项目的这一组成部分提供信息学支持和分析。在下一阶段，重点关注选择性剪接的结构影响，有五个主要目标：第一，开发一个数据库，纳入与高等真核生物，特别是人类的选择性剪接有关的广泛信息，以便能够选择最有趣和最有价值的案例进行实验研究。数据库的设计是基于我们在项目现阶段综合选择目标的信息的经验。第二，开发自动评估数据显示可靠性的工具 另一种拼接。在该领域发展的这个阶段，在进行实验工作之前，能够容易地评估支持拼接的数据是至关重要的。第三，制作结构模型，使人们能够直观地了解拼接对结构可能产生的影响。第四，开发一个全面的公共数据库，提供从我们自己和其他人工作中获得的关于结构背景下交替剪接的信息，并使用模型和实验结构。这一资源将使用户能够获得有关特定蛋白质的信息，并对剪接、结构、功能和疾病之间的一般关系进行分析。第五，分析剪接、结构和功能之间关系的工具的开发和应用。在本阶段开发的功能分析工具将构成这些工具的基础。

This component of the program project provides informatics support and analysis. In the next phase, focusing on the structural impact of alternative splicing, there are five principle goals: First, development of a database that integrates a wide range of information relevant to alternative splicing in higher eukaryotes, particularly human, so that the most interesting and rewarding cases can be selected for experimental study. The design of the database is based on our experience with integrating information for target choice in the present phase of the project. Second, development of tools for automatically assessing the reliability of data indicating alternative splicing. At this stage of the development of the field, it is critical to be able to easily assess the data supporting a splice, before committing experimental effort. Third, production of structure models, allowing a visual appreciation of the likely impact of splicing on structure. Fourth, development of a comprehensive public database that will provide information on alternate splicing in a structural context, obtained from our own and work that of others, and using both models and experimental structures. This resource will allow users to obtain information about specific proteins, and to perform analyses on the general relationship between splicing, structure, function and disease. Fifth, the development and application of tools to analyze the relationship between splicing, structure and function. Tools for the analysis of function developed in the present phase will form the basis of these.