FLS MEASUREMENTS OF SINGULAR MOLECULES

1. 使用膜探针Laurdan监测膜相。 月桂醇掺杂的T细胞的激发是使用 双光子激发和扫描整个T细胞， 完整的膜相位信息的三维细胞图。 2. 细胞内游离钙浓度用钙染法监测， fura 2. 钙标准品的初步结果已经完成 成功地 未来的工作包括三维扫描 含Fura的T细胞和3D游离Ca 细胞的浓度分布图。

1. Membrane phase was monitored using the membrane probe Laurdan. Excitation of the laurdan-doped T-cells was accomplished using two-photon excitation and scanning across the T-cell to develop a complete 3-dimensional cellular map of membrane phase information. 2. Cellular free Ca- concentrations were monitored using the calicurn dye fura 2. Preliminary results on calcium standards have been performed with success. Future work includes 3-dimensional scanning of fura-containing T-cells and reconstruction of a 3D , free Ca concentration map of the cell.