Direct sampling time resolved detector

直接采样时间分辨探测器

• 批准号: 7404366
• 负责人: Timothy M. Ragan
• 金额: $ 14.49万
• 依托单位: TISSUEVISION, INC.
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-15 至 2009-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7404366
• 关键词: Analog-Digital Conversion; Area; Cancer Biology; Caring; Cellular biology; Count; Data; Detection; Development; Diagnosis; Discipline; Dyes; Flow Cytometry; Fluorescence; Fluorescence Microscopy; Fluorescence Resonance Energy Transfer; Frequencies; Goals; Hybrids; Image; Light; Measurement; Memory; Methodology; Methods; Microscope; Neonatal; Noise; Pharmacologic Substance; Phase; Photons; Process; Protein Dynamics; Range; Rate; Sampling; Scanning; Semiconductors; Signal Transduction; Solutions; Source; Spectrum Analysis; Speed; Standards of Weights and Measures; Stream; Stroke; Techniques; Technology; Testing; Time; Tissues; analog; cancer diagnosis; cost; detector; drug development; high throughput screening; migration; noninvasive diagnosis; novel

DESCRIPTION (provided by applicant): The proposal describes the development of a novel method for frequency domain time resolved measurements. Application areas include fluorescence microscopy, fluorescence spectroscopy, and photon migration. The proposal is a hybrid approach that combines the sensitivity of time domain methods with the speed and dynamic range of the frequency domain. It has unqualified superiority over all current frequency domain fluorescence and photon migration techniques and rivals Time Correlated Single Photon Counting in sensitivity and signal to noise at low light levels while still be able to process high photon count rates. It will benefit a broad range of disciplines, including fluorescence lifetime imaging, fluorescence resonance energy transfer, photon migration, high throughput screening, and flow cytometry. This proposal will have broad impact in a range of biomedical applications from cancer biology, pharmaceutical development, cell biology, tissue imaging, and noninvasive diagnosis. This proposal describes the development of a new time resolved detection method which is significantly more sensitive and widely applicable than previous approaches. It will help areas such as neonatal care, stroke diagnosis, the study of protein dynamics, drug development and cancer diagnosis.

描述（由申请人提供）：该提案描述了一种用于频域时间分辨测量的新方法的开发。应用领域包括荧光显微镜、荧光光谱和光子迁移。该建议是一种混合方法，结合了时域方法的灵敏度与频域的速度和动态范围。它比目前所有的频域荧光和光子迁移技术都具有不合格的优越性，并且在低光水平下的灵敏度和信噪比方面与时间相关单光子计数相媲美，同时仍然能够处理高光子计数率。它将有利于广泛的学科，包括荧光寿命成像，荧光共振能量转移，光子迁移，高通量筛选和流式细胞术。该提案将在癌症生物学、药物开发、细胞生物学、组织成像和非侵入性诊断等一系列生物医学应用中产生广泛影响。该建议描述了一种新的时间分辨检测方法的发展，该方法比以前的方法更灵敏，适用范围更广。它将有助于新生儿护理、中风诊断、蛋白质动力学研究、药物开发和癌症诊断等领域。