CORE--ANIMAL

动物护理中心将提供动物护理，动物住房， 制备用于实验的SCID小鼠和异种移植肿瘤。的 核心活动还负责协调行政管理， 动物护理活动的监管报告和财务管理。 由于SCID小鼠是免疫抑制的并且含有人类肿瘤， 需要专门护理。所有动物手术均需无菌 技术和操作者需要专门培训。 动物护理中心的工作人员订购了SCID小鼠，每天记录 所有的肿瘤和动物，注射去瘤的人类黑色素瘤细胞， 受体异种移植物，监测原发性肿瘤异种移植物的生长， F1和F2代，根据治疗需要移植F2肿瘤 并准备异种移植物在零下95摄氏度下保存动物核心 工作人员负责准备所有监管记录， 动物资源部和美国动物保护协会。每日例行 提供动物的维护、饲养和设施卫生 作为动物资源部购买的服务。动物 最初被隔离在中央动物设施中，肿瘤 在生物安全中心动物设施接种和移植 水平2条件下，然后在无菌条件下转移至 用于监测肿瘤生长的实验室动物设施， 项目1、2、3或4的处理。 丹尼斯B。Leeper是核心总监，Judith A. Daviau研究所 兽医是副主任利珀医生将指导和监督 运营Daviau博士将在监测所有 手术，审查所有程序，确保所有人的健康和护理 动物和帮助改善和提升动物护理和手术 程序. Daviau博士的专长是实验室动物的护理。两 博士Daviau和Leeper博士在动物管理方面拥有丰富的经验 护理，包括SCID小鼠的菌落。利珀博士是 重症监护室Animal Care Core提供了来自 为项目1、2和3提供了所有的动物和肿瘤 项目4和与宾夕法尼亚大学的分包合同。 实验动物机构为A.A.A.L.A.C.。参阅会议过程

The Animal Care Core will provide animal care, animal housing and preparation of SCID mice and xenograft tumors for experimentation. The core activity is also responsible for coordinating the administration, regulatory reporting and fiscal management of animal care activities. Since SCID mice are immune suppressed and contain human tumors, specialized care is required. All animal procedures require sterile technique and handlers require specialized training. The Animal Care Core staff orders SCID mice, maintain the daily records on all of the tumors and animals, inject explanted human melanoma cells into recipient xenografts, monitor the growth of primary tumor xenografts in the F1 and F2 generations, transplant F2 tumors as required for treatment and prepare xenografts for -95 degrees Celsius storage. The Animal Core staff is responsible for preparation of all regulatory records for the Department of Animal Resources and the I.A.C.U.C. The routine daily maintenance, husbandry and facility hygiene for the animals are provided as a purchased service by the Department of Animal Resources. Animals are initially housed in isolation in the Central Animal Facility, tumors inoculated and transplanted in the Central Animal Facility under Biosafety Level 2 conditions and then transferred under sterile conditions to the laboratory animal facility for monitoring tumor growth for subsequent treatment in Projects 1, 2, 3 or 4. Dennis B. Leeper is the Core Director and Judith A. Daviau, Institute Veterinarian, is the Co-Director. Dr. Leeper will direct and oversee all operations. Dr. Daviau will have a critical role in monitoring all operations, reviewing all procedures, seeing to the health and care of all animals and helping improve and upgrade animal care and surgical procedures. Dr. Daviau's specialty is the care of laboratory animals. Both Dr. Daviau and Dr. Leeper have extensive experience supervising animal care, including colonies of SCID mice. Dr. Leeper is a member of the I.A.C.U.C. The Animal Care Core provides explanted melanoma cells from xenografts for Projects 1,2 and 3 and provides all the animals and tumors for Project 4 and the Subcontract with the University of Pennsylvania. The laboratory animal facility is A.A.A.L.A.C. approved.