Media for Capillary and Micro-Chip Based Electrophoresis

描述（由申请人提供）：更新提案的具体目标 涉及更好的分离介质的开发和优化， DNA毛细管/微芯片（阵列）电泳。一个概念模型， DNA链动力学和聚合物物理学的基本原理， 在聚合物合成的帮助下，已经开发并正在测试。我们 建议完成测试，设计新的分离介质， 模型，并将我们的分离介质付诸实践。 聚合物分离介质概念模型的实质是 开发和测试都非常好。基于该模型， 培养基应中性、亲水、稳定。它也应该有 表面活性特性。更重要的是，为了实现更快的运行 同时，聚合物链应该更多地延伸，使得相同的障碍物可以 用较少量的聚合物产生，这意味着较少量的 将需要聚合物来产生相同的有效筛目尺寸。更以 为了分离较大尺寸的碎片（例如，DNA;但不限于DNA 单独），聚合物链缠结时间应尽可能长（因此 以模拟化学交联的凝胶）。我们已经做的和将要做的 是通过使用一组 测试实验，因为有合成的限制，以创造一个理想的 分离介质此外，没有定量的理论治疗存在， 这种复杂的水平，在实践中，大多数聚合物不能很好地混合。因此，随着 这个新的概念模型，我们希望能够提供适当的手段， 为了改进任何现有的和未来的用于毛细管的分离介质， 电泳应用生物系统公司对我们的一个 容易获得的分离介质已经表明它比 ABI目前使用的商业分离介质。

DESCRIPTION (provided by applicant): The specific aim of the renewal proposal deals with the development and the optimization of better separation media for DNA capillary/microchip (array) electrophoresis. A conceptual model based on the DNA chain dynamics and fundamental principles of polymer physics, together with the aid of polymer synthesis, has been developed and is being tested. We propose to complete the tests, to design new separation media based on the model, and to put our separation media into practice. The essence of the conceptual model on polymer separation medium has been developed and tested out extremely well. Based on this model, the separation medium should be neutral, hydrophilic, and stable. It should also have surface-active properties. More importantly, in order to achieve a faster run time, the polymer chains should be more extended so that the same obstacles can be created with lesser amounts of polymers, implying that a lesser amount of polymers will be needed to create the same effective mesh size. Furthermore, in order to separate larger size fragments (e.g., DNAs; though not limited to DNAs alone), the polymer chain entanglement times should be as long as possible (so as to simulate a chemically cross-linked gel). What we have done and shall do is to demonstrate definitively the concepts of this model by using a set of test experiments since there are synthetic limitations to create an ideal separation medium. Furthermore, no quantitative theoretical treatment exists at this complex level, and in practice, most polymers do not mix well. Thus, with this new conceptual model, we hope to be able to provide the appropriate means to improve any existing and future separation media for capillary electrophoresis. Preliminary tests by Applied Biosystems on one of our more readily accessible separation media have already shown that it is better than the commercial separation medium currently being used by ABI.