CORE--CELL DISAGGREGATION AND CULTURE

核心III产生和维持细胞培养和分解的单细胞 为该项目的实施做准备。核心也对待 根据个别项目的要求进行准备，以满足以下方面的需要： 这些项目（如肽或儿茶酚胺的持续增长; 用特异性质粒进行腺病毒转染）。因此，核心服务于 许多功能。首先，它减轻了项目的成本， 维持和操作单个细胞解聚的时间， 文化设施。所有设备、用品和支助人员均 在物理上和管理上都集中起来。其次，它提供了 在规划实验性项目时， 围绕细胞培养和单细胞进行设计。第三，核心与 根据要求，开发和表征新细胞制剂的项目 不断发展的实验结果。

Core III produces and maintains cell culture and disaggregated single cell preparations for the Projects of this Program. The Core also treats preparations as requested by the individual Projects to serve the needs of those Projects (e.g. sustained growth in peptides or catecholamines; adenovirus transfection with specific plasmids). As such, the Core serves a number of functions. First, it relieves the Projects of the cost and time of maintaining and operating individual cell disaggregation and culture facilities. All equipment, supplies and supporting staff are centralized both physically and administratively. Second, it provides the projects with a source of technical expertise when planning experimental designs around cell cultures and single cells. Third, the Core works with Projects to develop and characterize new cell preparations, as required by evolving experimental results.