CORE--MORPHOLOGY

该核心是一个中央形态设施，为 该项目中的调查人员。提供的服务包括 常规光学显微镜处理，组织切片分析，以及 特殊的污渍。此外，在调查人员希望的地方， 透射电子显微镜、免疫荧光、 提供免疫组织化学和形态计量分析服务。 最后，该核心提供数字图像分析和图形 为调查人员提供插图支持。在过去，这个核心已经 使用量很大，预计需求将继续 增加。例如，在过去的一年里，超过2100个样本被 为计划项目的各个部分进行了处理。在这个总数中有 提交作光学显微镜分析的样本有1 640个，供化验的样本有305个 免疫组织化学和/或免疫荧光研究和181个样本 特姆。 我们预计，该核心的大量使用将由 未来几年的调查人员。例如，在第I、II、III、 和IV光学显微镜将被常规用于监测 不同模型的肺损伤情况。此外，体外研究还表明， 分离的细胞和组织培养单层将在所有部分进行 使用这种技术。TM研究也将在这方面得到广泛应用 所有部分都使用此技术的计划项目。 组织改变的形态计量学分析主要利用透射电子显微镜的遗嘱 在选定的组织上进行第二、第三和第四节。形态计量学 需要进行分析以准确地量化对肺的损伤 参数如肺泡和内皮细胞损伤百分比和 白细胞的定量和出血的常规评估 方法。此外，第二节将使用形态测量来评估大小 和肺内肉芽肿的分布。冰冻切片分析 组织和细胞，特别是免疫组织化学研究，是 目前被调查人员大量使用，这将在 目前的建议。免疫组织化学是用于 体内和体内黏附分子和细胞因子上调的评价 并将被该方案项目的所有部分所利用。 精选的免疫荧光研究也将使用冰冻 免疫复合体致肺损伤的切片评价及对肺损伤的影响 干预性研究主要在第一部分。最后，核心设施 提供数字图像分析和图形服务，包括彩色数字 激光打印海报和出版物，供调查人员使用 计划项目。

This core is a central morphology facility providing support for the investigators in the program project. The services provided include routine light microscopy processing, analysis of tissue sections, and special stains. In addition, where desired by the investigators, transmission electron microscopy (TEM), immunofluorescence, immunohistochemistry, and morphometric analysis services are available. Finally, this core provides digital image analysis and graphic illustration support for the investigators. In the past this core has received heavy usage and it is anticipated that demand will continue to increase. For example, during this past year over 2100 samples were processed for the sections of the program project. Of this total there were 1640 samples submitted for light microscopy analysis, 305 samples for immunohistochemistry and/or immunofluorescence studies and 181 samples for TEM. We anticipate that there will be heavy usage of this core by the investigators in the coming years. For example, in sections I, II, III, and IV light microscopy will be routinely used to monitor the intensity of the lung injury in various models. In addition, in vitro studies of isolated cells and tissue culture monolayers will be done in all sections using this technique. TEM studies will also be used extensively in this program project with all of the sections utilizing this technique. Morphometric analyses of tissue alterations utilizing primarily TEM will be done on selected tissues for sections II, III, and IV. Morphometric analysis is required to precisely quantitate injury to the lung with such parameters as percent alveolar and endothelial cell injury and quantitation of leukocytes and hemorrhage evaluated routinely by this method. In addition section II will use morphometry to evaluate the size and distribution of the granulomas in the lung. Frozen sections analysis of tissues and cells, particularly for immunohistochemistry studies, is heavily utilized currently by investigators and this will continue in the current proposal. Immunohistochemistry is the primary technique used for evaluating adhesion molecule and cytokine up-regulation in vivo and in vitro and will be utilized by all sections of the program project. Selected immunofluorescence studies will also be done utilizing frozen sections to evaluate immune complex induced lung injury and the effect of interventional studies primary in section 1. Finally, the core facility offers digital image analysis and graphic services including color digital laser printing for posters and publications for investigators in the program project.