EFFECTS OF SURFACE CHEMISTRY & PREADSORBED PROTEINS ON MONOCYTE ADHESION

在这项研究中，我们正在研究预吸附的效果， 单核细胞和巨噬细胞表面纤维蛋白原（Fg）和纤维连接蛋白（Fn 粘附到表面，以及细胞因子从吸附的 生物分子 粘附研究是在改良的96孔板上进行的。 由未处理的聚苯乙烯（PS）制成的聚苯乙烯微量滴定板， 氧合组织培养聚苯乙烯（TCPS）和其它商业上可接受的材料。 可用的盘子 用ESCA分析板的表面。 迄今为止的结果表明：1）单核细胞粘附和TNF（释放） 受表面化学和预吸附蛋白质的影响，2） TNF的量（释放的TNF与肿瘤细胞的数量不成正比） 粘附细胞，和3）吸附的纤维蛋白原促进释放 肿瘤坏死因子（TNF）

In this study, we are investigating the effect of preadsorbed fibrinogen (Fg) and fibronectin (Fn) on monocyte and macrophage adhesion to surfaces, and cytokine secretion from the adsorbed biomolecules. The adhesion studies are done on modified 96-well polystyrene microtiter plates made of untreated polystyrene (PS), oxygenated tissue culture polystyrene (TCPS), and other commercially available plates. The surface of the plates is analyzed with ESCA. Results to date indicate that 1) monocyte adhesion and TNF( release are affected by surface chemistry and pre-adsorbed proteins, 2) the amount of TNF( released is not directly proportional to the number of adherent cells, and 3) adsorbed fibrinogen promotes the release of TNF(..