TRIPLE QUADRUPOLE MASS SPECTROMETER

美国国立卫生研究院资助的七名研究人员要求购买一台带有电喷雾电离和大气压化学电离的三重四极杆质谱仪，以及一台液相色谱仪。该仪器将为华盛顿州立大学的核心小组和其他生物分子研究人员提供各种敏感和选择性定量LC/MS分析，串联质谱分析和一般LC/MS检测。该仪器将位于生物分析和生物技术实验室2单元，WSU的中央质谱设施。该仪器支持的研究项目包括P450介导反应产物的定量和表征；催化萜类底物环化的酶和突变酶的表征精子发生过程中体细胞和生殖细胞的基因表达生物活性muramyl肽的结构表征一种人蛋白与A.T特异性dna结合特性的研究氧化应激异前列腺素生物标志物的定量鉴定。

Funds to purchase a triple quadrupole mass spectrometer with electrospray ionization and atmospheric pressure chemical ionization, coupled to a liquid chromatograph, is requested by a group of seven NIH funded investigators. The instrument will provide capability for a variety of sensitive and selective quantitative LC/MS analyses, tandem mass spectrometry for structure elucidation, and general LC/MS detection for the core group and other biomolecular researchers at Washington State University. The instrument will be located in the Laboratory for Bioanalysis and Biotechnology, Unit 2, WSU's central MS facility. Research projects to be supported by this instrument include quantitation and characterization of products from P450 mediated reactions; characterization of enzymes and mutant enzymes which catalyze the cyclization of terpenoid substrates; gene expression in somatic and germ cells during spermatogenesis; structural characterization of biologically active muramyl peptides; investigation of A.T specific DNA-binding properties of a human protein; and quantitation and identification of isoprostane biomarkers of oxidative stress.