MICROARRAY EQUIPMENT

对发芽酵母酿酒酵母的基因组进行了测序。这种酵母提供了一个强大的遗传和分子系统。在这里，我们建议建立一个生产和使用酿酒酵母斑点DNA微阵列的设施。这些微阵列将主要用于纽约州立大学石溪分校的九个酵母实验室，如果资源允许，还将提供给美国其他地方的科学家。该设施将制造微阵列，并将提供数据库、软件和数据分析专业知识。该设施的外部用户将进行为微阵列分析设计的实验，制造RNA，验证RNA样本，并将RNA转换为标记的DNA。标记的c DNA将被送往设施，在那里它将与微阵列杂交，进行扫描，并接受标准形式的分析。结果将发布在一个网站上，供用户进一步分析。更广泛的数据分析资源将在该设施的网站上提供。该设施的运营将由用户向每个阵列实验收取约75美元的费用支持。

The genome of the budding yeast Saccharomyces cerevisiae has been sequenced. This yeast provides a powerful genetic and molecular system. Here, we propose to create a facility for the production and use of S. Cerevisiae spotted DNA micro arrays. These micro arrays would be made principally for thee community of nine yeast laboratories at the State University of New York, Stony Brook, and, if resources permit, to scientist elsewhere in the United States. The facility would manufacture micro arrays, and would provide a database, software, and expertise for the analysis of data. Outside users of the facility would do experiments designed for micro array analysis, make RNA, validate the RNA samples, and convert the RNA to labeled DNA. This labeled c DNA would be sent to the facility, where it wold be hybridized to the micro arrays, scanned, and subjected to standard forms of analysis. Results would be posted to a Web site for further analysis by users. Resources for more extensive data analysis would be available on the facility's Web site. The operation of the facility would be supported by user charges of about $75 per array experiment.