PHILIPS TECNAI 12 ELECTRON MICROSCOPE

此应用程序响应NIH计划公告PAR-99-031(NCRR共享仪器拨款)，并申请资金为耶鲁大学医学院妇产科购买替代的透射式电子显微镜。在过去的三十年里，在NIH的支持下，我们系的生殖神经科学小组一直在研究构成正常生殖和病理条件的基本中枢和外周机制，主要是使用形态学方法。我们也一直在与其他部门合作。透射式电子显微镜用于系内资助项目(80%)和合作项目(20%)。申请书中列出的12个项目使用了透射电子显微镜的超微结构分析。15年前，通过类似的NIH计划，我们购买了一台飞利浦CM 10电子显微镜。在此期间，新闻部支付了建造和维护电子显微镜套间和辅助设施的费用，并将继续这样做。虽然医学院和大学的其他部门还有其他电子显微镜，但由于PI以及Csaba Leranth博士和Tamas Horvath博士的所有项目都将电子显微镜作为主要的调查工具，我们小组几乎在工作日和周末的每一个小时都需要这些设备。因此，我们不可能在与其他部门共享的基础上解决我们对电子显微镜的需求。通过这一计划，我们正在申请购买一台新的电子显微镜：我们已有15年历史的飞利浦CM 10透射式电子显微镜贡献了已在数百篇同行评审研究文章中发表的观察结果，但它现在已完全损坏并停止使用。在过去的一年里，这台机器的大量使用导致故障数量不断增加。由于这一型号已经停产很久，很难找到合适的更换部件来重建显微镜。作为一种可行的工具，它已经被抛弃了，我们暂时使用了一台“封存的”过时的机器。但是，这个不能承受负荷，很快就会永远坏掉。列出了12个政府支持的项目(见正文)，这些项目总共需要经常使用电子显微镜。如果没有可能购买新的电子显微镜，这些项目就会岌岌可危！所有与会者的结论是，目前最友好和耐用的显微镜是飞利浦Tecnai 10，这是取代流行的飞利浦CM 100型号的型号。我们在正文中描述了飞利浦Tecnai 10。我们正在紧急申请资金，以购买这台机器和一个图像分析包，以支持我们由NIH资助的项目。

This application responds to NIH program announcement PAR-99-031 (NCRR shared instrumentation grant) and requests funding to purchase a replacement transmission electron microscope for the Department of Obstetrics and Gynecology, Yale University School of Medicine. For the last three decades, under NIH support, the reproductive neuroscience group in our department has been studying basic central and peripheral mechanisms that underlie normal reproduction and pathological conditions, primarily using morphological methods. We have also been collaborating with other departments. The transmission electron microscope is used for funded projects in the department (80%) plus collaborative projects (20%). The 12 projects listed in the application use ultrastructural analyses by transmission electron microscopy. Through a similar NIH initiative, we purchased a Philips CM 10 electron microscope 15 years ago. The Department has paid for the construction and maintenance of the electron microscopic suite and supporting facilities during this period, and will continue to do so. Although there are other electron microscopes in other departments of the Medical School and the University, because all the projects of the PI and those of Drs. Csaba Leranth and Tamas Horvath utilize electron microscopy as a primary tool of investigation our group has the need for the equipment almost every hour of the working day and on weekends. Thus, it is impossible to resolve our need of an electron microscope on a shared basis with other departments. Through this initiative, we are applying to purchase a new electron microscope: Our 15 year old Philips CM 10 transmission electron microscope contributed observations that have been published in hundreds of peer reviewed research articles, but it is now completely broken and out of service. The heavy use of the machine resulted in increasing numbers of breakdowns during the past year. Because this model has been long discontinued, it is difficult to find the appropriate replacement parts to rebuild the microscope. It has been abandoned as a viable instrument and we are temporarily using a "mothballed" obsolete machine. But, this one cannot withstand the load and will soon break down for good. Twelve government-supported projects are listed (see text) that in the aggregate require constant use of an electron microscope. Without the possibility of purchasing a new electron microscope, these projects are in jeopardy! It is the conclusion of all participants that the most user-friendly and durable microscope currently available is the Philips Tecnai 10, which is the model that replaced the popular Philips CM 100 model. We describe the Philips Tecnai 10 in the text. We are requesting funds urgently to purchase this machine and an image analysis package in order to support our NIH-funded projects.