PURCHASE OF A 600 MHZ NMR SPECTROMETER

本提案的目标是购买一台600 MHz NMR光谱仪， 韦恩州立大学在核糖体RNA领域正在进行的研究 结构和功能，B）RNA-蛋白质和RNA-抗生素复合物结构，C） DNA-致癌物加合物结构，D）α-1，3- 半乳糖基转移酶和参与金属转运和抗性的蛋白质，以及 E）神经元细胞裂解物中代谢物浓度的测定。这些主题 代表了六个完善的NIH资助实验室的研究。至少 新的600 MHz NMR将有85%的时间用于这些研究 项目剩余的仪器时间将用于其他活跃领域， 韦恩州立大学的研究包括：结合蛋白质的结构 肽模拟物，高分子化学，天然产物化学，机理 有机化学和RNase III-RNA相互作用。 韦恩州立大学唯一适合学习的乐器 生物分子的NMR是Varian UNITY 500 MHz NMR。这台仪器上的控制台 它已经过时，不能运行几个现代脉冲序列。此外，只有一半的 这台仪器上的大部分时间可用于生物分子的研究。添加 600 MHz NMR将大大扩展我们的NMR能力， 韦恩州立大学对高场核磁共振时间的需求不断增长 仪器增强的场强和改进的控制台和探头设计 将导致信噪比提高约30-40%， 减少光谱伪影，并将光谱分辨率提高20%， 维度（2D-NMR改进44%，3D-NMR改进73%）。 600 MHz核磁共振谱仪性能改进及可用性 更多的仪器时间将提高NMR数据的质量和数量。这 反过来将扩展我们研究更大生物分子结构的能力。 由于韦恩州立大学的团体数量大幅增加 进行生物分子核磁共振，一个严格的培训计划已经启动。 Ksebati博士对新用户进行正确使用NMR仪器的培训。 博士SantaLucia每周举行一次关于NMR理论和实践的会议， 一直有20多名学生和博士后研究员参加。 学生从初级NMR到高级理论进行培训，包括 核磁共振和脉冲序列的密度矩阵和乘积算子描述 编程.

The goal of this proposal is to purchase a 600 MHz NMR spectrometer to enhance ongoing research at Wayne State University in the areas of A) ribosomal RNA structure and function, B) RNA-protein and RNA-antibiotic complex structure, C) DNA-carcinogen adduct structure, D) protein structure determination of alpha-1,3- galactosyltransferase and proteins involved in metal transport and resistance, and E) assay of metabolite concentrations in neuronal cell lysates. These topics represent the research of six well-established NIH-funded laboratories. At least 85% of the time on the new 600 MHz NMR will be devoted to these research projects. The remaining instrument time will be devoted to other active areas of research at Wayne State University including: the structure of proteins with bound peptide mimics, polymer chemistry, natural products chemistry, mechanistic organic chemistry, and RNase III-RNA interactions. The only instrument at Wayne State University that is suitable for studying biomolecules is a Varian UNITY 500 MHz NMR. The console on this instrument is obsolete and it cannot run several modern pulse sequences. In addition, only half of the time on this instrument is available for studies of biomolecules. The addition of a 600 MHz NMR will significantly extend our NMR capabilities and will meet the growing demand at Wayne State University for time on a high-field NMR instrument. The increased field strength and improved console and probe design will result in a approximate 30-40% improvement in signal-to-noise ratio, will reduce spectral artifacts, and will improve spectral resolution by 20% in each dimension (44% improvement in 2D-NMR and 73% improvement in 3D-NMR). The improve performance of a 600 MHz NMR spectrometer and availability of more instrument time will increase both the quality and quantity of NMR data. This in turn will extend our ability to study the structures of larger biomolecules. Due to the large increase in the number of groups at Wayne State University performing biomolecular NMR, a rigorous training program has been initiated. New users are trained by Dr. Ksebati on the proper use of NMR instrumentation. Dr. SantaLucia holds weekly meetings on the theory and practice of NMR, which are consistently attended by more than 20 students and postdoctoral fellows. Students are trained from elementary NMR through advanced theory, including density matrix and product operator descriptions of NMR and pulse sequence programming.