MSC BLUE-3 AND MSC PURPLE-3 CONFOCAL X-RAY OPTICS

这份来自Fred Hutchinson癌症研究中心结构生物学项目的共享仪器提案要求为获得高性能、分级多层x射线光学器件提供资金。这些将更新和最大限度地提高现有的大分子晶体数据收集系统的能力，该系统由五个主要研究人员和他们的实验室使用。这一集成仪器系统的升级将极大地促进中心内部以及与全国大量分子生物学和生物化学实验室的持续合作。哈金森中心的大分子结构项目成立于大约七年前，是为了响应中心分子生物学实验室对协作结构/功能研究的明确需求，以支持他们在遗传调控、发育生物学、信号转导、细胞周期控制和其他基本生物现象方面的研究项目。此外，在结构酶学、时间分辨晶体学、病毒蛋白结构、蛋白质核酸识别、RNA结构、免疫系统识别与应答、蛋白质工程、晶体分相、蛋白质折叠等领域，均积极开展自己的原创性研究。因此，有必要最大限度地提高我们的x射线设备的能力，以满足我们对有效和准确的数据收集的要求。本提案的目标是通过用最先进的梯度多层x射线光学系统取代次优石墨单色器和弗兰克斯型反射镜，以经济有效地更新我们共享的衍射设备。我们正在申请资金购买MSC/Max-Flux共聚焦光学器件，这将使可用的x射线通量相对于目前的配置增加27倍。

This shared instrumentation proposal from the structural biology program at the Fred Hutchinson Cancer Research Center requests funding for the acquisition of high-performance, graded multilayer X-ray optics. These will update and maximize the capabilities of an existing macromolecular crystallographic data-collection system employed by five principal investigators and their laboratories. The upgrade of this integrated instrument system will greatly facilitate ongoing collaborative efforts both within the center and with a large number of molecular biology and biochemistry laboratories throughout the country. The macromolecular structure program at the Hutchinson Center was established approximately seven years ago as a response to a clear need among the molecular biology laboratories at the Center for collaborative structure/function studies which support their research programs in genetic regulation, developmental biology, signal transduction, cell cycle control, and other basic biological phenomena. In addition, all five principal investigators listed below actively conduct their own original research in such areas as structural enzymology, time-resolved crystallography, viral protein structure, protein-nucleic acid recognition, RNA structure, immune system recognition and response, protein engineering, crystallographic phasing, and protein folding. It is thus necessary to maximize the capabilities of our X-ray equipment to satisfy our requirements for efficient and accurate data collection. The goal of this proposal is to update cost-effectively our shared diffraction facility by replacing suboptimal graphite monochromator and Franks-type mirrors with state-of-the-art graded multilayer X-ray optics. We are requesting funds to purchase MSC/Max-Flux confocal optics that will result in up to 27-fold increase in useable X-ray flux, relative to the current configuration.