RNA TRAFFICKING IN SIMPLIFIED HIV1 DERIVATIVES

描述：（改编自研究者摘要）未剪接的逆转录病毒 RNA既用于翻译结构蛋白， 转化为病毒体。 尽管现在已经知道很多关于未剪接的 HIV RNA存在于细胞核中，其作用机制尚不清楚。 RNA在区室之间的运输用于翻译，或 一旦RNA到达细胞质，就会发生折叠。 本申请旨在 检验HIV-1未剪接RNA形成两种功能不同的 由病毒序列确定的样本池：一个样本池用于mRNA 翻译，和第二个池用于翻译。 本申请的第一个具体目的是表征 从简化的HIV-1基因组表达的RNA的代谢， 不同的元件，以促进未剪接的RNA的细胞质转运（如 作为Rev，来自MPMV的CTE，和HSV tk基因中的元件）。 第二 具体的目的是定义调节蛋白质的顺式和反式作用结构域， HIV-1 RNA在细胞质中的运输。

DESCRIPTION: (Adapted from Investigator's Abstract) Unspliced retroviral RNA is used for both translation of structural proteins and for encapsidation into virions. Although much is now known about how unspliced HIV RNA exists in the nucleus, little is known about the mechanism of trafficking of the RNA between the compartments for translation or encapsidation once the RNA reaches the cytoplasm. This application seeks to test the hypothesis that HIV-1 unspliced RNA forms two functionally distinct pools that are determined by viral sequences: one pool for mRNA translation, and a second pool for encapsidation. The first specific aim of this application is to characterize the metabolism of RNA expressed from simplified HIV-1 genomes that contain distinct elements to facilitate cytoplasmic transport of unspliced RNA (such as Rev, the CTE from MPMV, and an element in the HSV tk gene). The second specific aim is to define the cis and trans-acting domain that modulate trafficking of HIV-1 RNA in the cytoplasm.