课题基金 / 基金详情

MULTI PHOTON EXCITATION OF GREEN FLUORESCENT PROTEINS

MULTI PHOTON EXCITATION OF GREEN FLUORESCENT PROTEINS
绿色荧光蛋白的多光子激发
批准号:
6315380
负责人:
Joseph R. LAKOWICZ
金额:
$2.83万
依托单位国家:
美国
项目类别:
财政年份:
2000
资助国家:
美国
项目状态:
已结题
起止时间:
2000-04-01 至 2001-03-31

项目摘要

项目成果

Joseph R. LAKOWICZ的其他基金

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中文摘要
翻译
绿色荧光蛋白已广泛用于基因研究 表达,在细胞中,甚至在整个哺乳动物有机体中。 绿色荧光蛋白的双光子诱导荧光 突变体进行了观察和表征。 我们意外地发现 这个GFP突变体的双光子截面非常高, 比野生型GFP高几倍。 使用寿命和 用单光子和双光子激发测量各向异性衰减, 全球分析。 双光子激发的零时间各向异性 比单光子激发大10/7倍。
英文摘要
Green fluorescent proteins has become widely used to study gene expression, in cells and even in whole mammalian organisms. Two-Photon induced fluorescence of Green Fluorescence Protein (GFP) mutant was observed and characterized. Unexpectedly we have found that two-photon cross-section of this GFP mutant is enormously high, several time higher than for wild type GFP. The lifetime and anisotropy decays were measured with one and two photon excitation and analyzed globally. The zero time anisotropy for two photon excitation is large than for one photon excitation by the factor 10/7.
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Photonics-based Fluorescence Imaging for Research, Diagnostics, and Pathology
  • 批准号:
    10546493
  • 项目类别:
  • 资助金额:
    $38.63万
  • 财政年份:
    2022
  • 负责人:
    Joseph R. LAKOWICZ
  • 依托单位:
Photonics-based Fluorescence Imaging for Research, Diagnostics, and Pathology
  • 批准号:
    10329143
  • 项目类别:
  • 资助金额:
    $38.63万
  • 财政年份:
    2022
  • 负责人:
    Joseph R. LAKOWICZ
  • 依托单位:
Coupled Emission Microscopy for the Biosciences
  • 批准号:
    9424262
  • 项目类别:
  • 资助金额:
    $36.28万
  • 财政年份:
    2018
  • 负责人:
    Joseph R. LAKOWICZ
  • 依托单位:
Plasmon-coupled Fluorescence Correlation Spectroscopy in Nanoholes
  • 批准号:
    9766321
  • 项目类别:
  • 资助金额:
    $19.31万
  • 财政年份:
    2018
  • 负责人:
    Joseph R. LAKOWICZ
  • 依托单位: