CORE--NEUROANATOMY AND BEHAVIOR

神经解剖学核心将继续为包括该计划的六个项目中的每一个提供基本服务。该中心提供的服务将包括四个部分：1）标准组织学染色，包括Nissl、Weigart、Timms、Bodian和Fink-Heimer; 2）用于神经组织冷冻或石蜡切片中抗原免疫细胞化学定位的设备和试剂; 3）通过原位杂交定位mRNA种类的设施和设备;（4）电镜水平的超微结构研究设备和试剂，包括免疫电镜和定量分析。前三个组件已在Dodd博士的实验室中提供; EM研究在Bailey博士运营的第二个设施中进行。Dodd和Bailey博士在所采用的方法学方面具有丰富的相关经验。他们还将就试剂和设备的购买和使用作出决定，并将视需要经常举行会议，以评价核心小组提供的服务。详细说明了各个项目对核心服务的预期使用情况。行为核心将由霍金斯博士领导，并将继续提供各种行为测试，以探索转基因小鼠和不同品系野生型小鼠的学习能力进行比较。这些设施对六个项目中的每一个项目都作出了重大贡献。该核心目前有几个测试小鼠大脑皮层依赖性（外显）学习的设施（例如，Morris水迷宫、放射状臂迷宫、物体识别）。此外，核心具有内隐记忆的任务（例如，莫里斯水迷宫的暗示版本、味觉厌恶、气味增强惊吓）。最后，核心计划开发额外的测试，既依赖于校园学习，也独立于校园学习（例如，延迟不匹配的样本，视觉配对比较，和高阶方面的经典条件反射）。并非所有项目都利用这一核心，但考虑到中心的总体目标，这一点至关重要。这些是在过去五年中提供基础设施支持的持续核心。核心的理由是，集中这些职能应导致各项目技术的一致性和业务规模经济。这两个核心组成部分都符合中心的目标

The Neuroanatomical Core will continue to provide essential services for each of the six projects comprising this program. There will be four components of the services provided by the core: 1) standard histological stains, including Nissl, Weigart, Timms, Bodian, and Fink-Heimer; 2) equipment and reagents for the immunocytochemical localization of antigens in frozen or paraffin sections of neural tissue; 3) facilities and equipment for the localization of mRNA species by in situ hybridization; and 4) facilities and reagents for ultrastructural studies at the electron microscopic level, including immunoelectron microscopy and quantitative analyses. The first three of these components have been made available in the laboratory of Dr. Dodd; the EM studies are performed in a second facility operated by Dr. Bailey. Drs. Dodd and Bailey have extensive relevant experience in the methodologies to be employed. They will also make decisions on the purchase and use of reagents and equipment, and they will meet as often as is necessary to evaluate the services provided by the core. The expected use of the core services by individual projects is detailed. The Behavioral Core will be headed by Dr. Hawkins and will continue to provide a variety of behavioral tests to explore learning capabilities in transgenic mice and different strains of wild-type mice for comparison. These facilities have contributed significantly to each of the six projects. The core currently has facilities for several tests of hippocampal-dependent (explicit) learning in mice (e.g., Morris water maze, radial arm maze, object recognition). In addition, the core has tasks of implicit memory (e.g., a cued version of the Morris water maze, taste aversion, odor potentiated startle). Finally, the core plans to develop additional tests of both hippocampal-dependent and-independent learning (e.g., delayed non-match to sample, visual paired comparison, and higher-order aspects of classical conditioning). Not all projects make use of this core, but it is essential given the overall aims of the Center. These are continuing cores that have provided infrastructure support during the past five years. The rationale for the core is that centralizing these functions should lead to consistency in technology across projects and economies of scale of operation. Both of these core components are justified by the aims of the Center