CORE--CYTOMETRY FACILITY

在空间、时间和功能水平上分析单个细胞和细胞群体是现代免疫学、细胞生物学和发育生物学的核心。细胞学核心专注于提供光学显微镜或荧光激活细胞分类(FACS)技术，这些技术可以与特定的分子探针结合使用，如抗体、DNA、MHC-四聚体、细胞内报告分子，以解决与1型和2型糖尿病的发病机制、治疗和并发症有关的基本问题。核心中心将设在芭芭拉·戴维斯中心，由D·贝尔格鲁博士领导下的2名全职技术人员负责。它将使用最近从NIH获得的大型设备和慈善基金。将提供12项服务，包括标准的FACS分析和固体组织的免疫组织化学，以及对活细胞中的信号转导和亚细胞事件的更复杂的分析。将每两周至每月免费提供正式培训，并为所提供的每项服务提供专家咨询。

Analysis of individual cells and cell populations at the spatial, temporal and functional level lies at the heart of modern immunology, cell biology and developmental biology. The Cytometry Core focuses on the provision of light microscopal or fluorescence activated cell sorting (FACS) technologies which can be applied in combination with specific molecular probes such as antibodies, DNA, MHC-tetramers, intracellular reporter molecules to address fundamental questions relating to the pathogenesis, treatment and complications of type 1 and 2 diabetes. The Core will be located at the Barbara Davis Center and manned by 2 FTE technical staff under the director ship of Dr. D. Bellgrau. It will use items of large equipment recently obtained from NIH and charitable funding. Twelve services will be offered including standard FACS analyses and immunohistochemistry of solid tissues and more sophisticated analyses of signal transduction and subcellular events in living cells. Formal training will be offered without charge on a biweekly to monthly basis and expert consultation will be available for each of the services provided.